3mnm: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
m Protected "3mnm" [edit=sysop:move=sysop]
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{STRUCTURE_3mnm|  PDB=3mnm  |  SCENE=  }}
===Crystal structure of GAE domain of GGA2p from Saccharomyces cerevisiae===
{{ABSTRACT_PUBMED_020704189}}


==Function==
==Crystal structure of GAE domain of GGA2p from Saccharomyces cerevisiae==
[[http://www.uniprot.org/uniprot/GGA2_YEAST GGA2_YEAST]] May play a role in the regulation of membrane traffic through the trans-Golgi network.<ref>PMID:10747088</ref> <ref>PMID:11124697</ref>
<StructureSection load='3mnm' size='340' side='right'caption='[[3mnm]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3mnm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MNM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mnm OCA], [https://pdbe.org/3mnm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mnm RCSB], [https://www.ebi.ac.uk/pdbsum/3mnm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mnm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GGA2_YEAST GGA2_YEAST] May play a role in the regulation of membrane traffic through the trans-Golgi network.<ref>PMID:10747088</ref> <ref>PMID:11124697</ref>  
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mn/3mnm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mnm ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Different assemblies of accessory proteins with clathrin are critical for transporting precisely various cargos between intracellular compartments. GGA proteins are adaptors for clathrin-mediated intracellular trafficking, connecting other accessory and cargo proteins to clathrin-coated vesicles. Both binding to the GAE domain of GGA protein yGGA2 in Saccharomyces cerevisia, Ent3 and Ent5 are involved in different trafficking pathways. Ent5 is ubiquitous and localized in a manner independent of yGGA2, and Ent3 functions preferentially through yGGA2. Not known are the sources of these differences. Here we show not all acidic-phenylalanine motifs in Ent3/5 are active for yGGA2_GAE domain binding. Two of the three acidic-phenylalanine motifs from Ent3 can bind to the yGGA2_GAE domain, while only one of the two motifs from Ent5 can bind. We also determined the crystal structure of the yGGA2_GAE domain at 1.8 A resolution. Structural docking and mutagenesis analysis shows inactive motifs in Ent3 and Ent5 repel yGGA2_GAE binding through disfavored residues at positions 1 and 3. These results suggest accessory proteins may fine-tune the GGA adaptor dependence by adjusting their non-acidic-phenylalanine residues, thus contributing to the distinct role of Ent3 and Ent5 in trafficking.


==About this Structure==
Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 .,Fang P, Li X, Wang J, Niu L, Teng M Biochemistry. 2010 Sep 14;49(36):7949-55. PMID:20704189<ref>PMID:20704189</ref>
[[3mnm]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MNM OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<references group="xtra"/><references/>
</div>
<div class="pdbe-citations 3mnm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Fang, P.]]
[[Category: Fang P]]
[[Category: Li, X.]]
[[Category: Li X]]
[[Category: Niu, L.]]
[[Category: Niu L]]
[[Category: Teng, M.]]
[[Category: Teng M]]
[[Category: Wang, J.]]
[[Category: Wang J]]
[[Category: Beta sandwich]]
[[Category: Ig-like]]
[[Category: Protein transport]]
Retrieved from "https://proteopedia.org/3mnm"