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[[Image:2f23.gif|left|200px]]<br /><applet load="2f23" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2f23, resolution 1.60&Aring;" />
'''Crystal structure of GreA factor homolog 1 (Gfh1) protein of Thermus thermophilus'''<br />


==Overview==
==Crystal structure of GreA factor homolog 1 (Gfh1) protein of Thermus thermophilus==
Gfh1, a transcription factor from Thermus thermophilus, inhibits all catalytic activities of RNA polymerase (RNAP). We characterized the Gfh1 structure, function and possible mechanism of action and regulation. Gfh1 inhibits RNAP by competing with NTPs for coordinating the active site Mg2+ ion. This coordination requires at least two aspartates at the tip of the Gfh1 N-terminal coiled-coil domain (NTD). The overall structure of Gfh1 is similar to that of the Escherichia coli transcript cleavage factor GreA, except for the flipped orientation of the C-terminal domain (CTD). We show that depending on pH, Gfh1-CTD exists in two alternative orientations. At pH above 7, it assumes an inactive 'flipped' orientation seen in the structure, which prevents Gfh1 from binding to RNAP. At lower pH, Gfh1-CTD switches to an active 'Gre-like' orientation, which enables Gfh1 to bind to and inhibit RNAP.
<StructureSection load='2f23' size='340' side='right'caption='[[2f23]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2f23]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F23 FirstGlance]. <br>
2F23 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F23 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f23 OCA], [https://pdbe.org/2f23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f23 RCSB], [https://www.ebi.ac.uk/pdbsum/2f23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f23 ProSAT]</span></td></tr>
==Reference==
</table>
pH-dependent conformational switch activates the inhibitor of transcription elongation., Laptenko O, Kim SS, Lee J, Starodubtseva M, Cava F, Berenguer J, Kong XP, Borukhov S, EMBO J. 2006 May 17;25(10):2131-41. Epub 2006 Apr 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16628221 16628221]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/GFH1_THET2 GFH1_THET2] Inhibits all catalytic activities of RNA polymerase (RNAP) by partially occluding its substrate-binding site and preventing NTP binding.<ref>PMID:16628221</ref>
[[Category: Thermus thermophilus]]
== Evolutionary Conservation ==
[[Category: Kim, S S.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Kong, X P.]]
Check<jmol>
[[Category: crystal structure anti-grea gfh1 thermus thermophilus]]
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/2f23_consurf.spt"</scriptWhenChecked>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:16:55 2008''
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f23 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB27]]
[[Category: Kim S-S]]
[[Category: Kong XP]]

Latest revision as of 09:21, 14 February 2024

Crystal structure of GreA factor homolog 1 (Gfh1) protein of Thermus thermophilus

2f23, resolution 1.60Å

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