2f7t: Difference between revisions

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[[Image:2f7t.gif|left|200px]]<br /><applet load="2f7t" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2f7t, resolution 2.25&Aring;" />
'''Crystal structure of the catalytic domain of Mos1 mariner transposase'''<br />


==Overview==
==Crystal structure of the catalytic domain of Mos1 mariner transposase==
We present the crystal structure of the catalytic domain of Mos1 transposase, a member of the Tc1/mariner family of transposases. The structure comprises an RNase H-like core, bringing together an aspartic acid triad to form the active site, capped by N- and C-terminal alpha-helices. We have solved structures with either one Mg2+ or two Mn2+ ions in the active site, consistent with a two-metal mechanism for catalysis. The lack of hairpin-stabilizing structural motifs is consistent with the absence of a hairpin intermediate in Mos1 excision. We have built a model for the DNA-binding domain of Mos1 transposase, based on the structure of the bipartite DNA-binding domain of Tc3 transposase. Combining this with the crystal structure of the catalytic domain provides a model for the paired-end complex formed between a dimer of Mos1 transposase and inverted repeat DNA. The implications for the mechanisms of first and second strand cleavage are discussed.
<StructureSection load='2f7t' size='340' side='right'caption='[[2f7t]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2f7t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_mauritiana Drosophila mauritiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F7T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f7t OCA], [https://pdbe.org/2f7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f7t RCSB], [https://www.ebi.ac.uk/pdbsum/2f7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f7t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MOS1T_DROMA MOS1T_DROMA] Mediates transposition of transposon Mos1 by a 'cut and paste' mechanism. Transposases are sequence-specific nucleases and strand transferases that catalyze transposition through an ordered series of events: sequence-specific binding of transposase to the terminal inverted repeats (IR) present at each end of the transposon, pairing of the transposon IRs in a paired-end complex (PEC), cleavage of one or both DNA strands at each transposon end, capture of target DNA, and strand transfer to insert the transposon at a new site.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/2f7t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f7t ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2F7T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_mauritiana Drosophila mauritiana] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F7T OCA].
*[[Transposase 3D structures|Transposase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Mechanism of Mos1 transposition: insights from structural analysis., Richardson JM, Dawson A, O'Hagan N, Taylor P, Finnegan DJ, Walkinshaw MD, EMBO J. 2006 Mar 22;25(6):1324-34. Epub 2006 Mar 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511570 16511570]
[[Category: Drosophila mauritiana]]
[[Category: Drosophila mauritiana]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Dawson, A.]]
[[Category: Dawson A]]
[[Category: Finnegan, D J.]]
[[Category: Finnegan DJ]]
[[Category: Richardson, J M.]]
[[Category: Richardson JM]]
[[Category: Taylor, P.]]
[[Category: Taylor P]]
[[Category: Walkinshaw, M D.]]
[[Category: Walkinshaw MD]]
[[Category: MG]]
[[Category: ddd motif]]
[[Category: rnase-h like fold]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:38 2008''
Retrieved from "https://proteopedia.org/2f7t"