3no7: Difference between revisions
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== | ==Crystal structure of the centromere-binding protein ParB from plasmid pCXC100== | ||
[[3no7]] is a 2 chain structure with sequence from [ | <StructureSection load='3no7' size='340' side='right'caption='[[3no7]], [[Resolution|resolution]] 1.40Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3no7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leifsonia_xyli_subsp._cynodontis Leifsonia xyli subsp. cynodontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NO7 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3no7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3no7 OCA], [https://pdbe.org/3no7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3no7 RCSB], [https://www.ebi.ac.uk/pdbsum/3no7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3no7 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6EEF9_LEIXC Q6EEF9_LEIXC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Plasmid pCXC100 from the Gram-positive bacterium Leifsonia xyli subsp. cynodontis uses a type Ib partition system that includes a centromere region, a Walker-type ATPase ParA and a centromere-binding protein ParB for stable segregation. However, ParB shows no detectable sequence homology to any DNA-binding motif. Here, we study the ParB centromere interaction by structural and biochemical approaches. The crystal structure of the C-terminal DNA-binding domain of ParB at 1.4 A resolution reveals a dimeric ribbon-helix-helix (RHH) motif, supporting the prevalence of RHH motif in centromere binding. Using hydroxyl radical footprinting and quantitative binding assays, we show that the centromere core comprises nine uninterrupted 9-nt direct repeats that can be successively bound by ParB dimers in a cooperative manner. However, the interaction of ParB with a single subsite requires 18 base pairs covering one immediate repeat as well as two halves of flanking repeats. Through mutagenesis, sequence specificity was determined for each position of an 18-bp subsite. These data suggest an unique centromere recognition mechanism by which the repeat sequence is jointly specified by adjacent ParB dimers bound to an overlapped region. | |||
Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.,Huang L, Yin P, Zhu X, Zhang Y, Ye K Nucleic Acids Res. 2010 Dec 1. PMID:21123191<ref>PMID:21123191</ref> | |||
<ref | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3no7" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Leifsonia xyli subsp. cynodontis]] | [[Category: Leifsonia xyli subsp. cynodontis]] | ||
[[Category: Ye | [[Category: Ye K]] | ||
