3nj7: Difference between revisions

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~~{{STRUCTURE_3nj7| PDB=3nj7 | SCENE= }}~~

~~===1.9 A resolution X-ray structure of (GGCAGCAGCC)2===~~

~~{{ABSTRACT_PUBMED_20702420}}~~

==~~About this Structure~~==

==1.9 A resolution X-ray structure of (GGCAGCAGCC)2==

[[3nj7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NJ7 OCA].

== Structural highlights ==

<table><tr><td colspan='2'>[[3nj7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NJ7 FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.904Å</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nj7 OCA], [https://pdbe.org/3nj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nj7 RCSB], [https://www.ebi.ac.uk/pdbsum/3nj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nj7 ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

CAG repeats occur predominantly in the coding regions of human genes, which suggests their functional importance. In some genes, these sequences can undergo pathogenic expansions leading to neurodegenerative polyglutamine (poly-Q) diseases. The mutant transcripts containing expanded CAG repeats possibly contribute to pathogenesis in addition to the well-known pathogenic effects of mutant proteins. We have analysed two crystal forms of RNA duplexes containing CAG repeats: (GGCAGCAGCC)(2). One of the structures has been determined at atomic resolution (0.95 A) and the other at 1.9 A. The duplexes include non-canonical A-A pairs that fit remarkably well within a regular A-helix. All the adenosines are in the anti-conformation and the only interaction within each A-A pair is a single C2-H2...N1 hydrogen bond. Both adenosines in each A-A pair are shifted towards the major groove, although to different extents; the A which is the H-bond donor stands out more (the 'thumbs-up' conformation). The main effect on the helix conformation is a local unwinding. The CAG repeats and the previously examined CUG structures share a similar pattern of electrostatic charge distribution in the minor groove, which could explain their affinity for the pathogenesis-related MBNL1 protein.

~~==Reference==~~

Atomic resolution structure of CAG RNA repeats: structural insights and implications for the trinucleotide repeat expansion diseases.,Kiliszek A, Kierzek R, Krzyzosiak WJ, Rypniewski W Nucleic Acids Res. 2010 Aug 11. PMID:20702420<ref>PMID:20702420</ref>

~~[[Category: Kierzek, R.]]~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~[[Category: Kiliszek, A.]]~~

</div>

[[Category: ~~Krzyzosiak, W J.~~]]

[[Category: ~~Rypniewski, W.~~]]

== References ==

[[Category: ~~Cag repeat~~]]

[[Category: ~~Poly-q disease~~]]

__TOC__

[[Category: ~~Rna~~]]

</StructureSection>

[[Category: Large Structures]]

[[Category: Kierzek R]]

[[Category: Kiliszek A]]

[[Category: Krzyzosiak WJ]]

[[Category: Rypniewski W]]

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_3nj7|  PDB=3nj7  |  SCENE=  }}
-===1.9 A resolution X-ray structure of (GGCAGCAGCC)2===
-{{ABSTRACT_PUBMED_20702420}}
-==About this Structure==
+==1.9 A resolution X-ray structure of (GGCAGCAGCC)2==
-[[3nj7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NJ7 OCA].
+<StructureSection load='3nj7' size='340' side='right'caption='[[3nj7]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3nj7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NJ7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.904&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nj7 OCA], [https://pdbe.org/3nj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nj7 RCSB], [https://www.ebi.ac.uk/pdbsum/3nj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nj7 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+CAG repeats occur predominantly in the coding regions of human genes, which suggests their functional importance. In some genes, these sequences can undergo pathogenic expansions leading to neurodegenerative polyglutamine (poly-Q) diseases. The mutant transcripts containing expanded CAG repeats possibly contribute to pathogenesis in addition to the well-known pathogenic effects of mutant proteins. We have analysed two crystal forms of RNA duplexes containing CAG repeats: (GGCAGCAGCC)(2). One of the structures has been determined at atomic resolution (0.95 A) and the other at 1.9 A. The duplexes include non-canonical A-A pairs that fit remarkably well within a regular A-helix. All the adenosines are in the anti-conformation and the only interaction within each A-A pair is a single C2-H2...N1 hydrogen bond. Both adenosines in each A-A pair are shifted towards the major groove, although to different extents; the A which is the H-bond donor stands out more (the 'thumbs-up' conformation). The main effect on the helix conformation is a local unwinding. The CAG repeats and the previously examined CUG structures share a similar pattern of electrostatic charge distribution in the minor groove, which could explain their affinity for the pathogenesis-related MBNL1 protein.
-==Reference==
+Atomic resolution structure of CAG RNA repeats: structural insights and implications for the trinucleotide repeat expansion diseases.,Kiliszek A, Kierzek R, Krzyzosiak WJ, Rypniewski W Nucleic Acids Res. 2010 Aug 11. PMID:20702420<ref>PMID:20702420</ref>
-<ref group="xtra">PMID:020702420</ref><references group="xtra"/><references/>
-[[Category: Kierzek, R.]]
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Kiliszek, A.]]
+</div>
-[[Category: Krzyzosiak, W J.]]
+<div class="pdbe-citations 3nj7" style="background-color:#fffaf0;"></div>
-[[Category: Rypniewski, W.]]
+== References ==
-[[Category: Cag repeat]]
+<references/>
-[[Category: Poly-q disease]]
+__TOC__
-[[Category: Rna]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Kierzek R]]
+[[Category: Kiliszek A]]
+[[Category: Krzyzosiak WJ]]
+[[Category: Rypniewski W]]