2lms: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(6 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{STRUCTURE_2lms|  PDB=2lms  |  SCENE=  }}
===A single GalNAc residue on Threonine-106 modifies the dynamics and the structure of Interferon alpha-2a around the glycosylation site===
{{ABSTRACT_PUBMED_23184955}}


==Function==
==A single GalNAc residue on Threonine-106 modifies the dynamics and the structure of Interferon alpha-2a around the glycosylation site==
[[http://www.uniprot.org/uniprot/IFNA2_HUMAN IFNA2_HUMAN]] Produced by macrophages, IFN-alpha have antiviral activities.  
<StructureSection load='2lms' size='340' side='right'caption='[[2lms]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2lms]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LMS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lms OCA], [https://pdbe.org/2lms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lms RCSB], [https://www.ebi.ac.uk/pdbsum/2lms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lms ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzymatic addition of GalNAc to isotopically labeled IFNalpha2a produced in Escherichia coli yielded the O-linked glycoprotein GalNAcalpha-[(13)C,(15)N]IFNalpha2a. The three-dimensional structure of GalNAcalpha-IFNalpha2a has been determined in solution by NMR spectroscopy at high resolution. Proton-nitrogen heteronuclear Overhauser enhancement measurements revealed that the addition of a single monosaccharide unit at Thr-106 significantly slowed motions of the glycosylation loop on the nanosecond time scale. Subsequent addition of a Gal unit produced Gal(beta1,3)GalNAcalpha-[(13)C,(15)N]IFNalpha2a. This extension resulted in a further decrease in the dynamics of this loop. The methodology used here allowed the first such description of the structure and dynamics of an O-glycoprotein and opens the way to the study of this class of proteins.


==About this Structure==
A single N-acetylgalactosamine residue at threonine 106 modifies the dynamics and structure of interferon alpha2a around the glycosylation site.,Ghasriani H, Belcourt PJ, Sauve S, Hodgson DJ, Brochu D, Gilbert M, Aubin Y J Biol Chem. 2013 Jan 4;288(1):247-54. doi: 10.1074/jbc.M112.413252. Epub 2012, Nov 26. PMID:23184955<ref>PMID:23184955</ref>
[[2lms]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LMS OCA].
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2lms" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Interferon 3D structures|Interferon 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Aubin, Y.]]
[[Category: Large Structures]]
[[Category: Belcourt, P J.F.]]
[[Category: Aubin Y]]
[[Category: Brochu, D.]]
[[Category: Belcourt PJF]]
[[Category: Ghasriani, H.]]
[[Category: Brochu D]]
[[Category: Gilbert, M.]]
[[Category: Ghasriani H]]
[[Category: Gingras, G.]]
[[Category: Gilbert M]]
[[Category: Hodgson, D J.]]
[[Category: Gingras G]]
[[Category: Sauve, S.]]
[[Category: Hodgson DJ]]
[[Category: Cytokine]]
[[Category: Sauve S]]
[[Category: Glycoprotein]]
[[Category: Immune system]]
[[Category: O-glycosylation]]
[[Category: Signaling protein]]
[[Category: Type i interferon]]
Retrieved from "https://proteopedia.org/2lms"