4kcf: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 4kcf is ON HOLD Authors: Holden, H.M., Thoden, J.B., Branch, M.C., Zimmer, A.L., Bruender, N.A. Description: X-ray Structure of a KijD3 in Complex ... |
No edit summary |
||
| (6 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose== | ||
<StructureSection load='4kcf' size='340' side='right'caption='[[4kcf]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4kcf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinomadura_kijaniata Actinomadura kijaniata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KCF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.098Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKM:[(2R,4S,6R)-4-AZANYL-4,6-DIMETHYL-5,5-BIS(OXIDANYL)OXAN-2-YL]+[[(2R,3S,5R)-5-[5-METHYL-2,4-BIS(OXIDANYLIDENE)PYRIMIDIN-1-YL]-3-OXIDANYL-OXOLAN-2-YL]METHOXY-OXIDANYL-PHOSPHORYL]+HYDROGEN+PHOSPHATE'>AKM</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kcf OCA], [https://pdbe.org/4kcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kcf RCSB], [https://www.ebi.ac.uk/pdbsum/4kcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kcf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B3TMR1_ACTKI B3TMR1_ACTKI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
KijD3 is a flavin-dependent N-oxygenase implicated in the formation of the nitro-containing sugar d-kijanose, found attached to the antibiotic kijanimicin. For this investigation, the structure of KijD3 in complex with FMN and its dTDP-sugar substrate was solved to 2.1 A resolution. In contrast to the apoenzyme structure, the C-terminus of the protein becomes ordered and projects into the active site cleft [Bruender, N. A., Thoden, J. B., and Holden, H. M. (2010) Biochemistry 49, 3517-3524]. The amino group of the dTDP-aminosugar that is oxidized is located 4.9 A from C4a of the flavin ring. The model provides a molecular basis for understanding the manner in which KijD3 catalyzes its unusual chemical transformation. | |||
Active Site Architecture of a Sugar N-Oxygenase.,Thoden JB, Branch MC, Zimmer AL, Bruender NA, Holden HM Biochemistry. 2013 Apr 30. PMID:23621882<ref>PMID:23621882</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4kcf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Actinomadura kijaniata]] | |||
[[Category: Large Structures]] | |||
[[Category: Branch MC]] | |||
[[Category: Bruender NA]] | |||
[[Category: Holden HM]] | |||
[[Category: Thoden JB]] | |||
[[Category: Zimmer AL]] | |||
Latest revision as of 15:57, 20 September 2023
X-ray Structure of a KijD3 in Complex with FMN and dTDP-3-amino-2,3,6-trideoxy-4-keto-3-methyl-D-glucose
| ||||||||||||
