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[[Image:2fi7.gif|left|200px]]<br /><applet load="2fi7" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2fi7, resolution 2.2&Aring;" />
'''Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa'''<br />


==Overview==
==Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa==
PilF is a requisite protein involved in the type 4 pilus biogenesis system from the Gram-negative human pathogenic bacteria, Pseudomonas aeruginosa. We determined the PilF structure at a 2.2A resolution; this includes six tandem tetratrico peptide repeat (TPR) units forming right-handed superhelix. PilF structure was similar to the heat shock protein organizing protein, which interacts with the C-terminal peptide of Hsp90 and Hsp70 via a concave Asn ladder in the inner groove of TPR superhelix. After simulated screening, the C-terminal pentapeptides of PilG, PilU, PilY, and PilZ proved to be a likely candidate binding to PilF, which are ones of 25 necessary components involved in the type 4 pilus biogenesis system. We proposed that PilF would be critical as a bridgehead in protein-protein interaction and thereby, PilF may bind a necessary molecule in type 4 pilus biogenesis system such as PilG, PilU, PilY, and PilZ.
<StructureSection load='2fi7' size='340' side='right'caption='[[2fi7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2fi7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FI7 FirstGlance]. <br>
2FI7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FI7 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fi7 OCA], [https://pdbe.org/2fi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fi7 RCSB], [https://www.ebi.ac.uk/pdbsum/2fi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fi7 ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of PilF: functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa., Kim K, Oh J, Han D, Kim EE, Lee B, Kim Y, Biochem Biophys Res Commun. 2006 Feb 24;340(4):1028-38. Epub 2005 Dec 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16403447 16403447]
== Function ==
[[Category: Pseudomonas aeruginosa]]
[https://www.uniprot.org/uniprot/PILF_PSEAE PILF_PSEAE] Essential component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers (PubMed:8973346). Plays an essential role in the outer membrane localization and assembly of PilQ into secretins which are dodecamers of PilQ (PubMed:23547883, PubMed:18776008).<ref>PMID:18776008</ref> <ref>PMID:23547883</ref> <ref>PMID:8973346</ref>
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Kim, K.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: crystal structure]]
Check<jmol>
[[Category: pilf]]
  <jmolCheckbox>
[[Category: pseudomonas aeruginosa]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fi/2fi7_consurf.spt"</scriptWhenChecked>
[[Category: tpr]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: type 4 pilus]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:21:39 2008''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fi7 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Kim K]]

Latest revision as of 13:50, 13 March 2024

Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

2fi7, resolution 2.20Å

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