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[[Image:2gcs.gif|left|200px]]<br /><applet load="2gcs" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2gcs, resolution 2.10&Aring;" />
'''Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme'''<br />


==Overview==
==Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme==
The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.
<StructureSection load='2gcs' size='340' side='right'caption='[[2gcs]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2gcs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GCS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2AD:2-AMINO-2-DEOXYADENOSINE'>2AD</scene>, <scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gcs OCA], [https://pdbe.org/2gcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gcs RCSB], [https://www.ebi.ac.uk/pdbsum/2gcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gcs ProSAT]</span></td></tr>
</table>


==About this Structure==
==See Also==
2GCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GCS OCA].
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990543 16990543]
[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Amare, A R.Ferre-D.]]
[[Category: Ferre-D'Amare AR]]
[[Category: Klein, D J.]]
[[Category: Klein DJ]]
[[Category: MG]]
[[Category: rna; ribozyme; pseudoknot; helix]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:30:27 2008''

Latest revision as of 09:27, 14 February 2024

Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme

2gcs, resolution 2.10Å

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