3q1p: Difference between revisions

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{{STRUCTURE_3q1p|  PDB=3q1p  |  SCENE=  }}
===Crystal structure of CDP-Chase===
{{ABSTRACT_PUBMED_21531795}}


==About this Structure==
==Crystal structure of CDP-Chase==
[[3q1p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1P OCA].  
<StructureSection load='3q1p' size='340' side='right'caption='[[3q1p]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3q1p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_14579 Bacillus cereus ATCC 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q1P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q1P FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q1p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q1p OCA], [https://pdbe.org/3q1p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q1p RCSB], [https://www.ebi.ac.uk/pdbsum/3q1p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q1p ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q81EE8_BACCR Q81EE8_BACCR]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A Nudix enzyme from Bacillus cereus (NCBI RefSeq accession no. NP_831800) catalyzes the hydrolysis of CDP-choline to produce CMP and phosphocholine. Here, we show that in addition, the enzyme has a 3'--&gt;5' RNA exonuclease activity. The structure of the free enzyme, determined to a 1.8-A resolution, shows that the enzyme is an asymmetric dimer. Each monomer consists of two domains, an N-terminal helical domain and a C-terminal Nudix domain. The N-terminal domain is placed relative to the C-terminal domain such as to result in an overall asymmetric arrangement with two distinct catalytic sites: one with an "enclosed" Nudix pyrophosphatase site and the other with a more open, less-defined cavity. Residues that may be important for determining the asymmetry are conserved among a group of uncharacterized Nudix enzymes from Gram-positive bacteria. Our data support a model where CDP-choline hydrolysis is catalyzed by the enclosed Nudix site and RNA exonuclease activity is catalyzed by the open site. CDP-Chase is the first identified member of a novel Nudix family in which structural asymmetry has a profound effect on the recognition of substrates.


==Reference==
The Nudix Hydrolase CDP-Chase, a CDP-Choline Pyrophosphatase, Is an Asymmetric Dimer with Two Distinct Enzymatic Activities.,Duong-Ly KC, Gabelli SB, Xu W, Dunn CA, Schoeffield AJ, Bessman MJ, Amzel LM J Bacteriol. 2011 Jul;193(13):3175-85. Epub 2011 Apr 29. PMID:21531795<ref>PMID:21531795</ref>
<ref group="xtra">PMID:021531795</ref><references group="xtra"/><references/>
 
[[Category: Bacillus cereus]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Amzel, L M.]]
</div>
[[Category: Duong-Ly, K C.]]
<div class="pdbe-citations 3q1p" style="background-color:#fffaf0;"></div>
[[Category: Gabelli, S B.]]
== References ==
[[Category: Asymmetric dimer]]
<references/>
[[Category: Cdp-choline pyrophosphatase]]
__TOC__
[[Category: Hydrolase]]
</StructureSection>
[[Category: Nudix]]
[[Category: Bacillus cereus ATCC 14579]]
[[Category: Rna exonuclease]]
[[Category: Large Structures]]
[[Category: Amzel LM]]
[[Category: Duong-Ly KC]]
[[Category: Gabelli SB]]
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