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{{STRUCTURE_4go1|  PDB=4go1  |  SCENE=  }}
===Crystal Structure of full length transcription repressor LsrR from E. coli.===
{{ABSTRACT_PUBMED_23589368}}


==About this Structure==
==Crystal Structure of full length transcription repressor LsrR from E. coli.==
[[4go1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GO1 OCA].  
<StructureSection load='4go1' size='340' side='right'caption='[[4go1]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4go1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GO1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4go1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4go1 OCA], [https://pdbe.org/4go1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4go1 RCSB], [https://www.ebi.ac.uk/pdbsum/4go1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4go1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LSRR_ECOLI LSRR_ECOLI] Regulates transcription of many different genes. In the absence of autoinducer 2 (AI-2), represses transcription of the lsrACDBFG operon and its own transcription. In the presence of AI-2, LsrR is inactivated by binding phospho-AI-2, leading to the transcription of the lsr genes.<ref>PMID:15601708</ref> <ref>PMID:15743955</ref> <ref>PMID:17557827</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Quorum-sensing systems are widely used by bacteria to control behavior in response to fluctuations in cell density. Several small diffusible molecules called autoinducers act as signaling molecules in quorum-sensing processes through interplay with sensors. Autoinducers modulate vital physiological functions such as nutrient acquisition, gene transcription, and virulence factor production. In Escherichia coli, LsrR serves as a global transcription regulator that responds to autoinducer-2 to regulate the expression of a variety of genes, including the lsr operon and the lsrR gene. Here, we report the crystal structure of full-length LsrR from E. coli, which has an N-terminal DNA-binding domain and a C-terminal ligand-binding domain connected by a beta-strand. Although only two molecules are found in one asymmetric unit, two neighboring dimers pack to form a tetramer that is consistent with the oligomerization state of LsrR in solution. Mutagenesis experiments and gel shift assays indicated that Gln-33 and Tyr-26 might be involved in interactions between LsrR and DNA. The LsrR-binding site for phosphorylated autoinducer-2 was predicted by structural comparisons of LsrR with CggR and SorC. Cross-linking, size exclusion chromatography, and gel shift assays determined that phosphorylated autoinducer-2 triggered the disassembly of the LsrR tetramer into dimers and reduced the DNA binding ability of LsrR. Our findings reveal a mechanism for the change in the oligomerization state of LsrR in the presence of phosphorylated autoinducer-2. Based on these observations, we propose that phosphorylated autoinducer-2 triggers the disassembly of the LsrR tetramer to activate the transcription of its target genes.


==Reference==
Structural Basis for Phosphorylated Autoinducer-2 Modulation of the Oligomerization State of the Global Transcription Regulator LsrR from Escherichia coli.,Wu M, Tao Y, Liu X, Zang J J Biol Chem. 2013 May 31;288(22):15878-87. doi: 10.1074/jbc.M112.417634. Epub, 2013 Apr 15. PMID:23589368<ref>PMID:23589368</ref>
<ref group="xtra">PMID:023589368</ref><references group="xtra"/><references/>
 
[[Category: Escherichia coli k-12]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Liu, X.]]
</div>
[[Category: Tao, Y.]]
<div class="pdbe-citations 4go1" style="background-color:#fffaf0;"></div>
[[Category: Wu, M.]]
== References ==
[[Category: Zang, J.]]
<references/>
[[Category: Hth motif]]
__TOC__
[[Category: P-ai-2]]
</StructureSection>
[[Category: Sorc/deor family]]
[[Category: Escherichia coli K-12]]
[[Category: Transcription]]
[[Category: Large Structures]]
[[Category: Transcription repressor]]
[[Category: Liu X]]
[[Category: Tao Y]]
[[Category: Wu M]]
[[Category: Zang J]]
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