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[[Image:2hqn.jpg|left|200px]]<br /><applet load="2hqn" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2hqn" />
'''Structure of a Atypical Orphan Response Regulator Protein Revealed a New Phosphorylation-Independent Regulatory Mechanism'''<br />


==Overview==
==Structure of a Atypical Orphan Response Regulator Protein Revealed a New Phosphorylation-Independent Regulatory Mechanism==
Two-component signal transduction systems, commonly found in prokaryotes, typically regulate cellular functions in response to environmental conditions through a phosphorylation-dependent process. A new type of response regulator, hp1043 (HP-RR) from Helicobacter pylori, has been recently identified. HP-RR is essential for cell growth and does not require the well known phosphorelay scheme. Unphosphorylated HP-RR binds specifically to its own promoter (P(1043)) and autoregulates the promoter of the tlpB gene (P(tlpB)). We have determined the structure of HP-RR by NMR and x-ray crystallography, revealing a symmetrical dimer with two functional domains. The molecular topology resembles that of the OmpR/PhoB subfamily, however, the symmetrical dimer is stable even in the unphosphorylated state. The dimer interface, formed by three secondary structure elements (alpha4-beta5-alpha5), resembles that of the active, phosphorylated forms of ArcA and PhoB. Several conserved residues of the HP-RR dimeric interface deviate from the OmpR/PhoB subfamily, although there are similar salt bridges and hydrophobic patches within the interface. Our findings reveal how a new type of response regulator protein could function as a cell growth-associated regulator in the absence of post-translational modification.
<StructureSection load='2hqn' size='340' side='right'caption='[[2hqn]]' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2hqn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HQN FirstGlance]. <br>
2HQN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQN OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hqn OCA], [https://pdbe.org/2hqn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hqn RCSB], [https://www.ebi.ac.uk/pdbsum/2hqn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hqn ProSAT]</span></td></tr>
==Reference==
</table>
Structure of an atypical orphan response regulator protein supports a new phosphorylation-independent regulatory mechanism., Hong E, Lee HM, Ko H, Kim DU, Jeon BY, Jung J, Shin J, Lee SA, Kim Y, Jeon YH, Cheong C, Cho HS, Lee W, J Biol Chem. 2007 Jul 13;282(28):20667-75. Epub 2007 May 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17491010 17491010]
== Function ==
[[Category: Helicobacter pylori]]
[https://www.uniprot.org/uniprot/Q9ZM42_HELPJ Q9ZM42_HELPJ]
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Hong, E.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Lee, W.]]
Check<jmol>
[[Category: phosporylation-independent response regulator]]
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/2hqn_consurf.spt"</scriptWhenChecked>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:44:39 2008''
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hqn ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Helicobacter pylori J99]]
[[Category: Large Structures]]
[[Category: Hong E]]
[[Category: Lee W]]
Retrieved from "https://proteopedia.org/2hqn"