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{{STRUCTURE_3vmf|  PDB=3vmf  |  SCENE=  }}
===Archaeal protein===
{{ABSTRACT_PUBMED_22772989}}


==Function==
==Archaeal protein==
[[http://www.uniprot.org/uniprot/EF1A_AERPE EF1A_AERPE]] This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity). [[http://www.uniprot.org/uniprot/RF1_AERPE RF1_AERPE]] Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA (By similarity).  
<StructureSection load='3vmf' size='340' side='right'caption='[[3vmf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3vmf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix_K1 Aeropyrum pernix K1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VMF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vmf OCA], [https://pdbe.org/3vmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vmf RCSB], [https://www.ebi.ac.uk/pdbsum/3vmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vmf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EF1A_AERPE EF1A_AERPE] This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
When a stop codon appears at the ribosomal A site, the class I and II release factors (RFs) terminate translation. In eukaryotes and archaea, the class I and II RFs form a heterodimeric complex, and complete the overall translation termination process in a GTP-dependent manner. However, the structural mechanism of the translation termination by the class I and II RF complex remains unresolved. In archaea, archaeal elongation factor 1 alpha (aEF1alpha), a carrier GTPase for tRNA, acts as a class II RF by forming a heterodimeric complex with archaeal RF1 (aRF1). We report the crystal structure of the aRF1.aEF1alpha complex, the first active class I and II RF complex. This structure remarkably resembles the tRNA.EF-Tu complex, suggesting that aRF1 is efficiently delivered to the ribosomal A site, by mimicking tRNA. It provides insights into the mechanism that couples GTP hydrolysis by the class II RF to stop codon recognition and peptidyl-tRNA hydrolysis by the class I RF. We discuss the different mechanisms by which aEF1alpha recognizes aRF1 and aPelota, another aRF1-related protein and molecular evolution of the three functions of aEF1alpha.


==About this Structure==
Structural basis for translation termination by archaeal RF1 and GTP-bound EF1alpha complex.,Kobayashi K, Saito K, Ishitani R, Ito K, Nureki O Nucleic Acids Res. 2012 Jul 5. PMID:22772989<ref>PMID:22772989</ref>
[[3vmf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeropyrum_pernix_k1 Aeropyrum pernix k1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VMF OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:022772989</ref><references group="xtra"/><references/>
</div>
[[Category: Aeropyrum pernix k1]]
<div class="pdbe-citations 3vmf" style="background-color:#fffaf0;"></div>
[[Category: Ishitani, R.]]
 
[[Category: Ito, K.]]
==See Also==
[[Category: Kobayashi, K.]]
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
[[Category: Nureki, O.]]
== References ==
[[Category: Saito, K.]]
<references/>
[[Category: Translation]]
__TOC__
[[Category: Translation termination]]
</StructureSection>
[[Category: Aeropyrum pernix K1]]
[[Category: Large Structures]]
[[Category: Ishitani R]]
[[Category: Ito K]]
[[Category: Kobayashi K]]
[[Category: Nureki O]]
[[Category: Saito K]]
Retrieved from "https://proteopedia.org/3vmf"