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{{STRUCTURE_4dwx|  PDB=4dwx  |  SCENE=  }}
===Crystal Structure of a Family GH-19 Chitinase from rye seeds===
{{ABSTRACT_PUBMED_22831795}}


==Function==
==Crystal Structure of a Family GH-19 Chitinase from rye seeds==
[[http://www.uniprot.org/uniprot/CHIC_SECCE CHIC_SECCE]] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.<ref>PMID:11999399</ref> <ref>PMID:11826968</ref> <ref>PMID:12092848</ref>
<StructureSection load='4dwx' size='340' side='right'caption='[[4dwx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4dwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Secale_cereale Secale cereale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DWX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dwx OCA], [https://pdbe.org/4dwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dwx RCSB], [https://www.ebi.ac.uk/pdbsum/4dwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dwx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CHIC_SECCE CHIC_SECCE] Defense against chitin containing fungal pathogens. Binds the hyphal tips of fungi and degrades nascent chitin.<ref>PMID:11999399</ref> <ref>PMID:11826968</ref> <ref>PMID:12092848</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Substrate-binding mode of a 26-kDa GH19 chitinase from rye, Secale cereale, seeds (RSC-c) was investigated by crystallography, site-directed mutagenesis, and NMR spectroscopy. The crystal structure of RSC-c in a complex with an N-acetylglucosamine tetramer, (GlcNAc)(4) , was successfully solved, and revealed the binding mode of the tetramer to an aglycon binding site, subsites +1, +2, +3, and +4. This is the first crystallographic data showing the oligosaccharide-binding mode of a family GH-19 chitinase. From an HPLC analysis of the enzymatic reaction products, mutation of Trp72 to alanine was found to affect the product distribution obtained from the substrate, p-nitrophenyl penta-N-acetyl-beta-chitopentaoside. The mutational experiments confirmed the crystallographic finding that the Trp72 side chain interacts with the +4 moiety of the bound substrate. To further confirm the crystallographic data, the binding experiments were also conducted in solution using NMR spectroscopy. Several signals in the (1) H-(15) N HSQC spectrum of the stable isotope-labeled RSC-c were affected upon the addition of (GlcNAc)(4) . Signal assignments revealed that most signals responsive to the addition of (GlcNAc)(4) are derived from amino acids located at the surface of the aglycon-binding site. The binding mode deduced from NMR binding experiments in solution was well consistent with that from the crystal structure. (c) 2012 The Authors Journal compilation (c) 2012 FEBS.


==About this Structure==
Crystal structure and chitin oligosaccharide-binding mode of a "loopful" family GH19 chitinase from rye, Secale cereal, seeds.,Ohnuma T, Numata T, Osawa T, Inanaga H, Okazaki Y, Shinya S, Kondo K, Fukuda T, Fukamizo T FEBS J. 2012 Jul 25. doi: 10.1111/j.1742-4658.2012.08723.x. PMID:22831795<ref>PMID:22831795</ref>
[[4dwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Secale_cereale Secale cereale]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DWX OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:022831795</ref><references group="xtra"/><references/>
</div>
[[Category: Chitinase]]
<div class="pdbe-citations 4dwx" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Chitinase 3D structures|Chitinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Secale cereale]]
[[Category: Secale cereale]]
[[Category: Fukamizo, T.]]
[[Category: Fukamizo T]]
[[Category: Numata, T.]]
[[Category: Numata T]]
[[Category: Ohnuma, T.]]
[[Category: Ohnuma T]]
[[Category: Umemoto, N.]]
[[Category: Umemoto N]]
[[Category: Carbohydrate]]
[[Category: Chitinase]]
[[Category: Hydrolase]]
Retrieved from "https://proteopedia.org/4dwx"