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[[Image:2hwk.gif|left|200px]]<br /><applet load="2hwk" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2hwk, resolution 2.45&Aring;" />
'''Crystal Structure of Venezuelan Equine Encephalitis Alphavirus nsP2 Protease Domain'''<br />


==Overview==
==Crystal Structure of Venezuelan Equine Encephalitis Alphavirus nsP2 Protease Domain==
Alphavirus replication and propagation is dependent on the protease activity of the viral nsP2 protein, which cleaves the nsP1234 polyprotein replication complex into functional components. Thus, nsP2 is an attractive target for drug discovery efforts to combat highly pathogenic alphaviruses. Unfortunately, antiviral development has been hampered by a lack of structural information for the nsP2 protease. Here, we report the crystal structure of the nsP2 protease (nsP2pro) from Venezuelan equine encephalitis alphavirus determined at 2.45 A resolution. The protease structure consists of two distinct domains. The nsP2pro N-terminal domain contains the catalytic dyad cysteine and histidine residues organized in a protein fold that differs significantly from any known cysteine protease or protein folds. The nsP2pro C-terminal domain displays structural similarity to S-adenosyl-L-methionine-dependent RNA methyltransferases and provides essential elements that contribute to substrate recognition and may also regulate the structure of the substrate binding cleft.
<StructureSection load='2hwk' size='340' side='right'caption='[[2hwk]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2hwk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Venezuelan_equine_encephalitis_virus_(strain_TC-83) Venezuelan equine encephalitis virus (strain TC-83)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HWK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hwk OCA], [https://pdbe.org/2hwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hwk RCSB], [https://www.ebi.ac.uk/pdbsum/2hwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hwk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9WIJ1_EEVVT Q9WIJ1_EEVVT]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/2hwk_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hwk ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2HWK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Venezuelan_equine_encephalitis_virus Venezuelan equine encephalitis virus] with <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HWK OCA].
*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The crystal structure of the Venezuelan equine encephalitis alphavirus nsP2 protease., Russo AT, White MA, Watowich SJ, Structure. 2006 Sep;14(9):1449-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16962975 16962975]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Russo AT]]
[[Category: Venezuelan equine encephalitis virus]]
[[Category: Watowich SJ]]
[[Category: Russo, A T.]]
[[Category: White MA]]
[[Category: Watowich, S J.]]
[[Category: White, M A.]]
[[Category: FMT]]
[[Category: alpha/beta/alpha]]
[[Category: multi-domain]]
[[Category: rossman fold]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:46:31 2008''

Latest revision as of 09:34, 14 February 2024

Crystal Structure of Venezuelan Equine Encephalitis Alphavirus nsP2 Protease Domain

2hwk, resolution 2.45Å

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