4blf: Difference between revisions
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== | ==Variable internal flexibility characterizes the helical capsid formed by Agrobacterium VirE2 protein on single-stranded DNA.== | ||
[[http://www.uniprot.org/uniprot/ | <SX load='4blf' size='340' side='right' viewer='molstar' caption='[[4blf]], [[Resolution|resolution]] 20.00Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4blf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BLF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 20Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4blf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blf OCA], [https://pdbe.org/4blf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4blf RCSB], [https://www.ebi.ac.uk/pdbsum/4blf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4blf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/VIRE2_AGRFC VIRE2_AGRFC] Involved in DNA transformation; mediates the nuclear uptake of single-stranded DNA copies of the transferred DNA (T-DNA) element. Binds single-stranded but not double-stranded DNA regardless of nucleotide sequence composition.<ref>PMID:12124400</ref> <ref>PMID:17784072</ref> <ref>PMID:8637884</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Agrobacterium is known for gene transfer to plants. In addition to a linear ssDNA oligonucleotide, Agrobacterium tumefaciens secretes an abundant ssDNA-binding effector, VirE2. In many ways VirE2 adapts the conjugation mechanism to transform the eukaryotic host. The crystal structure of VirE2 shows two compact domains joined by a flexible linker. Bound to ssDNA, VirE2 forms an ordered solenoidal shell, or capsid known as the T-complex. Here, we present a three-dimensional reconstruction of the VirE2-ssDNA complex using cryo-electron microscopy and iterative helical real-space reconstruction. High-resolution refinement was not possible due to inherent heterogeneity in the protein structure. By a combination of computational modeling, chemical modifications, mass spectroscopy, and electron paramagnetic resonance, we found that the N-terminal domain is tightly constrained by both tangential and longitudinal links, while the C terminus is weakly constrained. The quaternary structure is thus rigidly assembled while remaining locally flexible. This flexibility may be important in accommodating substrates without sequence specificity. | |||
Variable Internal Flexibility Characterizes the Helical Capsid Formed by Agrobacterium VirE2 Protein on Single-Stranded DNA.,Bharat TA, Zbaida D, Eisenstein M, Frankenstein Z, Mehlman T, Weiner L, Sorzano CO, Barak Y, Albeck S, Briggs JA, Wolf SG, Elbaum M Structure. 2013 Jun 11. pii: S0969-2126(13)00157-3. doi:, 10.1016/j.str.2013.04.027. PMID:23769668<ref>PMID:23769668</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
< | </div> | ||
<div class="pdbe-citations 4blf" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[VirE1-VirE2|VirE1-VirE2]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Agrobacterium tumefaciens]] | [[Category: Agrobacterium tumefaciens]] | ||
[[Category: Albeck | [[Category: Large Structures]] | ||
[[Category: Barak | [[Category: Albeck S]] | ||
[[Category: Bharat | [[Category: Barak Y]] | ||
[[Category: Briggs | [[Category: Bharat TAM]] | ||
[[Category: Eisenstein | [[Category: Briggs JAG]] | ||
[[Category: Elbaum | [[Category: Eisenstein M]] | ||
[[Category: Frankenstein | [[Category: Elbaum M]] | ||
[[Category: Mehlman | [[Category: Frankenstein Z]] | ||
[[Category: Sorzano | [[Category: Mehlman T]] | ||
[[Category: Weiner | [[Category: Sorzano COS]] | ||
[[Category: Wolf | [[Category: Weiner L]] | ||
[[Category: Zbaida | [[Category: Wolf SG]] | ||
[[Category: Zbaida D]] | |||
Latest revision as of 11:01, 9 May 2024
Variable internal flexibility characterizes the helical capsid formed by Agrobacterium VirE2 protein on single-stranded DNA.
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