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{{STRUCTURE_3rip|  PDB=3rip  |  SCENE=  }}
===Crystal Structure of human gamma-tubulin complex protein 4 (GCP4)===
{{ABSTRACT_PUBMED_21725292}}


==Function==
==Crystal Structure of human gamma-tubulin complex protein 4 (GCP4)==
[[http://www.uniprot.org/uniprot/GCP4_HUMAN GCP4_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.  
<StructureSection load='3rip' size='340' side='right'caption='[[3rip]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3rip]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rip OCA], [https://pdbe.org/3rip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rip RCSB], [https://www.ebi.ac.uk/pdbsum/3rip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rip ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCP4_HUMAN GCP4_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microtubule nucleation in all eukaryotes involves gamma-tubulin small complexes (gammaTuSCs) that comprise two molecules of gamma-tubulin bound to gamma-tubulin complex proteins (GCPs) GCP2 and GCP3. In many eukaryotes, multiple gammaTuSCs associate with GCP4, GCP5 and GCP6 into large gamma-tubulin ring complexes (gammaTuRCs). Recent cryo-EM studies indicate that a scaffold similar to gammaTuRCs is formed by lateral association of gammaTuSCs, with the C-terminal regions of GCP2 and GCP3 binding gamma-tubulin molecules. However, the exact role of GCPs in microtubule nucleation remains unknown. Here we report the crystal structure of human GCP4 and show that its C-terminal domain binds directly to gamma-tubulin. The human GCP4 structure is the prototype for all GCPs, as it can be precisely positioned within the gammaTuSC envelope, revealing the nature of protein-protein interactions and conformational changes regulating nucleation activity.


==About this Structure==
Crystal structure of gamma-tubulin complex protein GCP4 provides insight into microtubule nucleation.,Guillet V, Knibiehler M, Gregory-Pauron L, Remy MH, Chemin C, Raynaud-Messina B, Bon C, Kollman JM, Agard DA, Merdes A, Mourey L Nat Struct Mol Biol. 2011 Jul 3;18(8):915-9. doi: 10.1038/nsmb.2083. PMID:21725292<ref>PMID:21725292</ref>
[[3rip]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIP OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:021725292</ref><references group="xtra"/><references/>
</div>
<div class="pdbe-citations 3rip" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Gregory-Pauron, L.]]
[[Category: Large Structures]]
[[Category: Guillet, V.]]
[[Category: Gregory-Pauron L]]
[[Category: Mourey, L.]]
[[Category: Guillet V]]
[[Category: Gamma-tubulin ring complex]]
[[Category: Mourey L]]
[[Category: Gamma-turc]]
[[Category: Helix bundle]]
[[Category: Structural protein]]
Retrieved from "https://proteopedia.org/3rip"