3weo: Difference between revisions
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New page: '''Unreleased structure''' The entry 3weo is ON HOLD Authors: Tagami, Takayoshi, Yamashita, Keitaro, Okuyama, Masayuki, Mori, Haruhide, Yao, Min, Kimura, Atsuo Description: sugar beet ... |
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The | ==Sugar beet alpha-glucosidase with acarviosyl-maltohexaose== | ||
<StructureSection load='3weo' size='340' side='right'caption='[[3weo]], [[Resolution|resolution]] 1.45Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3weo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Beta_vulgaris Beta vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WEO FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3weo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3weo OCA], [https://pdbe.org/3weo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3weo RCSB], [https://www.ebi.ac.uk/pdbsum/3weo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3weo ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/L0N7E5_BETVU L0N7E5_BETVU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The alpha-glucosidase from sugar beet (SBG) is an exo-type glycosidase. The enzyme has a pocket-shaped active site, but efficiently hydrolyzes longer maltooligosaccharides and soluble starch due to lower Km and higher kcat/Km for such substrates. To obtain structural insights into the mechanism governing its unique substrate specificity, a series of acarviosyl-maltooligosaccharides was employed for steady-state kinetic and structural analyses. The acarviosyl-maltooligosaccharides have a longer maltooligosaccharide moiety compared to the maltose moiety of acarbose, which is known to be the transition-state analog of alpha-glycosidases. The clear correlation obtained between logKi of the acarviosyl-maltooligosaccharides and log(Km/kcat) for hydrolysis of maltooligosaccharides suggests that the acarviosyl-maltooligosaccharides are transition state mimics. The crystal structure of the enzyme bound with acarviosyl-maltohexaose reveals that substrate binding at a distance from the active site is maintained largely by van der Waals interactions, with the four glucose residues at the reducing terminus of acarviosyl-maltohexaose retaining a left-handed single-helical conformation, as also observed in cycloamyloses and single-helical V-amyloses. The kinetic behavior and structural features suggest that the subsite structure suitable for the stable conformation of amylose lowers the Km for long-chain substrates, which in turn is responsible for higher specificity of the longer substrates. | |||
Structural advantage of sugar beet alpha-glucosidase to stabilize the Michaelis complex with long-chain substrate.,Tagami T, Yamashita K, Okuyama M, Mori H, Yao M, Kimura A J Biol Chem. 2014 Dec 1. pii: jbc.M114.606939. PMID:25451917<ref>PMID:25451917</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3weo" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Beta vulgaris]] | |||
[[Category: Large Structures]] | |||
[[Category: Kimura A]] | |||
[[Category: Mori H]] | |||
[[Category: Okuyama M]] | |||
[[Category: Tagami T]] | |||
[[Category: Yamashita K]] | |||
[[Category: Yao M]] | |||
Latest revision as of 13:07, 8 November 2023
Sugar beet alpha-glucosidase with acarviosyl-maltohexaose
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