2mb4: Difference between revisions
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New page: '''Unreleased structure''' The entry 2mb4 is ON HOLD Authors: Adrian, M., Lech, C., Phan, A. Description: Solution structure of a stacked dimeric G-quadruplex formed by a segment of th... |
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The | ==Solution structure of a stacked dimeric G-quadruplex formed by a segment of the human CEB1 minisatellite== | ||
<StructureSection load='2mb4' size='340' side='right'caption='[[2mb4]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2mb4]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MB4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mb4 OCA], [https://pdbe.org/2mb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mb4 RCSB], [https://www.ebi.ac.uk/pdbsum/2mb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mb4 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CEB1 is a highly polymorphic human minisatellite. In yeast, the size variation of CEB1 tandem arrays has been associated with the capacity of the motif to form G-quadruplexes. Here we report on the NMR solution structure of a G-quadruplex formed by the CEB1 DNA G-rich fragment d(AGGGGGGAGGGAGGGTGG), harboring several G-tracts including one with six continuous guanines. This sequence forms a dimeric G-quadruplex involving the stacking of two subunits, each being a unique snapback parallel-stranded scaffold with three G-tetrad layers, three double-chain-reversal loops, and a V-shaped loop. The two subunits are stacked at their 5'-end tetrads, and multiple stacking rotamers may be present due to a high symmetry at the stacking interface. There is a conformational exchange in the millisecond time scale involving a swapping motion between two bases of the six-guanine tract. Our results not only add to the understanding of how the G-quadruplex formation in human minisatellite leads to genetic instability but also address the fundamental questions regarding stacking of G-quadruplexes and how a long continuous G-tract participates in the structure and conformational dynamics of G-quadruplexes. | |||
Structure and Conformational Dynamics of a Stacked Dimeric G-Quadruplex Formed by the Human CEB1 Minisatellite.,Adrian M, Ang DJ, Lech CJ, Heddi B, Nicolas A, Phan AT J Am Chem Soc. 2014 Apr 30;136(17):6297-305. doi: 10.1021/ja4125274. Epub 2014, Apr 17. PMID:24742225<ref>PMID:24742225</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2mb4" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Adrian M]] | |||
[[Category: Ang DJ]] | |||
[[Category: Heddi B]] | |||
[[Category: Lech C]] | |||
[[Category: Nicolas A]] | |||
[[Category: Phan AT]] | |||
Latest revision as of 06:03, 15 May 2024
Solution structure of a stacked dimeric G-quadruplex formed by a segment of the human CEB1 minisatellite
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