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{{STRUCTURE_4bw9|  PDB=4bw9  |  SCENE=  }}
===PylRS Y306G, Y384F, I405R mutant in complex with AMP-PNP===


==Function==
==PylRS Y306G, Y384F, I405R mutant in complex with AMP-PNP==
[[http://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA]] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).  
<StructureSection load='4bw9' size='340' side='right'caption='[[4bw9]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4bw9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BW9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bw9 OCA], [https://pdbe.org/4bw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bw9 RCSB], [https://www.ebi.ac.uk/pdbsum/4bw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bw9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Three for two: By using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase.


==About this Structure==
Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins.,Schneider S, Gattner MJ, Vrabel M, Flugel V, Lopez-Carrillo V, Prill S, Carell T Chembiochem. 2013 Sep 11. doi: 10.1002/cbic.201300435. PMID:24027216<ref>PMID:24027216</ref>
[[4bw9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanosarcina_mazei Methanosarcina mazei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BW9 OCA].
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4bw9" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanosarcina mazei]]
[[Category: Methanosarcina mazei]]
[[Category: Carell, T.]]
[[Category: Carell T]]
[[Category: Fluegel, V.]]
[[Category: Fluegel V]]
[[Category: Gattner, M J.]]
[[Category: Gattner MJ]]
[[Category: Lopez-Carillo, V.]]
[[Category: Lopez-Carillo V]]
[[Category: Schneider, S.]]
[[Category: Schneider S]]
[[Category: Vrabel, M.]]
[[Category: Vrabel M]]
[[Category: Ligase]]
Retrieved from "https://proteopedia.org/4bw9"