3wcq: Difference between revisions
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== | ==Crystal structure analysis of Cyanidioschyzon melorae ferredoxin D58N mutant== | ||
[[3wcq]] is a 1 chain structure with sequence from [ | <StructureSection load='3wcq' size='340' side='right'caption='[[3wcq]], [[Resolution|resolution]] 0.97Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3wcq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyanidioschyzon_merolae Cyanidioschyzon merolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WCQ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wcq OCA], [https://pdbe.org/3wcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wcq RCSB], [https://www.ebi.ac.uk/pdbsum/3wcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wcq ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q85FT5_CYAM1 Q85FT5_CYAM1] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.[RuleBase:RU364001] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40-50 degrees C) and acidic (pH 1-3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe-2S] Fd by physicochemical techniques. A high resolution (0.97A) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method to clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP(+) reductase or cytochrome c. | |||
Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation - A high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant.,Ueno Y, Matsumoto T, Yamano A, Imai T, Morimoto Y Biochem Biophys Res Commun. 2013 Jul 12;436(4):736-9. doi:, 10.1016/j.bbrc.2013.06.029. Epub 2013 Jun 18. PMID:23792094<ref>PMID:23792094</ref> | |||
<ref | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3wcq" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cyanidioschyzon merolae]] | [[Category: Cyanidioschyzon merolae]] | ||
[[Category: Imai | [[Category: Large Structures]] | ||
[[Category: Matsumoto | [[Category: Imai T]] | ||
[[Category: Morimoto | [[Category: Matsumoto T]] | ||
[[Category: Ueno | [[Category: Morimoto Y]] | ||
[[Category: Yamano | [[Category: Ueno Y]] | ||
[[Category: Yamano A]] | |||
