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{{STRUCTURE_3w1e|  PDB=3w1e  |  SCENE=  }}
===Structure of FlgT, a flagellar basal body component protein===
{{ABSTRACT_PUBMED_23530206}}


==About this Structure==
==Structure of FlgT, a flagellar basal body component protein==
[[3w1e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_alginolyticus Vibrio alginolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W1E OCA].  
<StructureSection load='3w1e' size='340' side='right'caption='[[3w1e]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3w1e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_alginolyticus Vibrio alginolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W1E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w1e OCA], [https://pdbe.org/3w1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w1e RCSB], [https://www.ebi.ac.uk/pdbsum/3w1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w1e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D9N551_VIBAL D9N551_VIBAL]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Flagellar motility is a key factor for bacterial survival and growth in fluctuating environments. The polar flagellum of a marine bacterium, Vibrio alginolyticus, is driven by sodium ion influx and rotates approximately six times faster than the proton-driven motor of Escherichia coli. The basal body of the sodium motor has two unique ring structures, the T ring and the H ring. These structures are essential for proper assembly of the stator unit into the basal body and to stabilize the motor. FlgT, which is a flagellar protein specific for Vibrio sp., is required to form and stabilize both ring structures. Here, we report the crystal structure of FlgT at 2.0-A resolution. FlgT is composed of three domains, the N-terminal domain (FlgT-N), the middle domain (FlgT-M), and the C-terminal domain (FlgT-C). FlgT-M is similar to the N-terminal domain of TolB, and FlgT-C resembles the N-terminal domain of FliI and the alpha/beta subunits of F1-ATPase. To elucidate the role of each domain, we prepared domain deletion mutants of FlgT and analyzed their effects on the basal-body ring formation. The results suggest that FlgT-N contributes to the construction of the H-ring structure, and FlgT-M mediates the T-ring association on the LP ring. FlgT-C is not essential but stabilizes the H-ring structure. On the basis of these results, we propose an assembly mechanism for the basal-body rings and the stator units of the sodium-driven flagellar motor.


==Reference==
Insight into the assembly mechanism in the supramolecular rings of the sodium-driven Vibrio flagellar motor from the structure of FlgT.,Terashima H, Li N, Sakuma M, Koike M, Kojima S, Homma M, Imada K Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):6133-8. doi:, 10.1073/pnas.1222655110. Epub 2013 Mar 25. PMID:23530206<ref>PMID:23530206</ref>
<ref group="xtra">PMID:023530206</ref><references group="xtra"/><references/>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3w1e" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio alginolyticus]]
[[Category: Vibrio alginolyticus]]
[[Category: Homma, M.]]
[[Category: Homma M]]
[[Category: Imada, K.]]
[[Category: Imada K]]
[[Category: Sakuma, M.]]
[[Category: Sakuma M]]
[[Category: Terashima, H.]]
[[Category: Terashima H]]
[[Category: H-ring formation]]
[[Category: Motor protein]]
[[Category: The flagellar basal body]]
Retrieved from "https://proteopedia.org/3w1e"