Luciola cruciata luciferase: Difference between revisions
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<StructureSection load='2d1r' size=' | <StructureSection load='2d1r' size='350' side='right' caption='Structure of Luciola cruciata complexed with Oxiluciferin and AMP (PDB entry [[2d1r]])' scene='Luciola_cruciata_luciferase/Oxyluciferin_amp/2' > | ||
[[Luciferase]] is a class of enzymes producing light through the process of bioluminescence. [[Luciola cruciata luciferase]], or Japanese firefly luciferase, catalyses a reaction of adenylation and then an oxydative decarboxylation, changing luciferin to oxyluciferin and thus emitting light. | [[Luciferase]] is a class of enzymes producing light through the process of bioluminescence. [[Luciola cruciata luciferase]], or Japanese firefly luciferase, catalyses a reaction of adenylation and then an oxydative decarboxylation, changing luciferin to oxyluciferin and thus emitting light. | ||
This protein is constituted of two main domains separated by a cleft. | This protein is constituted of two main domains separated by a cleft. | ||
==Chemical reaction== | ==Chemical reaction== | ||
The enzyme catalyses the production of light, using two different conformation to catalyze two half-reactions (in the case of firefly Luciferase). | The enzyme catalyses the production of light, using two different conformation to catalyze two half-reactions (in the case of firefly Luciferase). | ||
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It can also be used to study the action of general anesthetics<ref name="ane" />, which are inhibiting it. | It can also be used to study the action of general anesthetics<ref name="ane" />, which are inhibiting it. | ||
The [http://en.wikipedia.org/wiki/Luciferase#Applications applications for luciferase] are very diverse. | The [http://en.wikipedia.org/wiki/Luciferase#Applications applications for luciferase] are very diverse. | ||
==Biology== | ==Biology== | ||
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==3D structures of luciferase== | ==3D structures of luciferase== | ||
[[Luciferase]] | [[Luciferase]] | ||
