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{{STRUCTURE_4hna|  PDB=4hna  |  SCENE=  }}
===Kinesin motor domain in the ADP-MG-ALFX state in complex with tubulin and a DARPIN===
{{ABSTRACT_PUBMED_23872990}}


==Function==
==Kinesin motor domain in the ADP-MG-ALFX state in complex with tubulin and a DARPIN==
[[http://www.uniprot.org/uniprot/KINH_HUMAN KINH_HUMAN]] Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).  
<StructureSection load='4hna' size='340' side='right'caption='[[4hna]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4hna]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HNA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.19&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hna OCA], [https://pdbe.org/4hna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hna RCSB], [https://www.ebi.ac.uk/pdbsum/4hna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hna ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D0VWZ0_SHEEP D0VWZ0_SHEEP] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[RuleBase:RU003505][SAAS:SAAS023123_004_019801]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The typical function of kinesins is to transport cargo along microtubules. Binding of ATP to microtubule-attached motile kinesins leads to cargo displacement. To better understand the nature of the conformational changes that lead to the power stroke that moves a kinesin's load along a microtubule, we determined the X-ray structure of human kinesin-1 bound to alphabeta-tubulin. The structure defines the mechanism of microtubule-stimulated ATP hydrolysis, which releases the kinesin motor domain from microtubules. It also reveals the structural linkages that connect the ATP nucleotide to the kinesin neck linker, a 15-amino acid segment C terminal to the catalytic core of the motor domain, to result in the power stroke. ATP binding to the microtubule-bound kinesin favors neck-linker docking. This biases the attachment of kinesin's second head in the direction of the movement, thus initiating each of the steps taken.


==About this Structure==
Structure of a kinesin-tubulin complex and implications for kinesin motility.,Gigant B, Wang W, Dreier B, Jiang Q, Pecqueur L, Pluckthun A, Wang C, Knossow M Nat Struct Mol Biol. 2013 Aug;20(8):1001-7. doi: 10.1038/nsmb.2624. Epub 2013 Jul, 21. PMID:23872990<ref>PMID:23872990</ref>
[[4hna]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Alfaropsis_roxburghiana Alfaropsis roxburghiana], [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNA OCA].
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4hna" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Tubulin|Tubulin]]
*[[Kinesin 3D Structures|Kinesin 3D Structures]]
 
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
==Reference==
== References ==
<ref group="xtra">PMID:023872990</ref><references group="xtra"/><references/>
<references/>
[[Category: Alfaropsis roxburghiana]]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Ovis aries]]
[[Category: Ovis aries]]
[[Category: Synthetic construct]]
[[Category: Synthetic construct]]
[[Category: Gigant, B.]]
[[Category: Gigant B]]
[[Category: Knossow, M.]]
[[Category: Knossow M]]
[[Category: Alpha-tubulin]]
[[Category: Beta-tubulin]]
[[Category: Darpin]]
[[Category: Gtpase]]
[[Category: Kinesin]]
[[Category: Microtubule]]
[[Category: Motor protein]]
[[Category: Tubulin]]
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