Adaptin: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
Michal Harel (talk | contribs)
32,820 edits
No edit summary
Michal Harel (talk | contribs)
32,820 edits
No edit summary
 
(22 intermediate revisions by 2 users not shown)
Line 1: Line 1:
<StructureSection load='1dq8' size='350' side='right' caption='Structure of HMG-CoA reductase (PDB entry [[1dq8]])' scene=''>
<StructureSection load='2vgl' size='350' side='right' caption='Structure of Adaptin core: AP-α-2 (cyan), AP-β-1 (magenta), AP-μ-1 (green), AP-σ-1 (olive) complex with inositole hexakisphosphate  (PDB entry [[2vgl]])' scene='55/559108/Cv/1'>
'''Adaptin''' (AP) are proteins which mediate the formation of vescicles by clathrin-coated pits. The AP complex is a hetertetramer composed of 2 large AP: AP-α or AP-γ and AP-β, a medium-size AP: AP-μ and small AP: AP-σ.  AP complexes connect proteins and lipids to clathrin budding sites.  There are different AP complexes in mammals.
'''Adaptin''' (AP) are proteins which mediate the formation of vescicles by clathrin-coated pits.<br />
The '''AP-2 complex''' is a heterotetramer composed a core domain containing 2 '''large AP''': AP-α or AP-γ and AP-β, a '''medium-size AP''': AP-μ and '''small AP''': AP-σ, 2 appendage domains or '''EAR'''.  The core domain binds to the membrane and to cargo proteins which should be internalized and the EAR domains bind to clathrin and accessory proteins.  There are different AP complexes in mammals.<ref name="Ad">PMID:12086608</ref>
*<scene name='55/559108/Cv/3'>Adaptin core: AP-α-2, AP-β-1, AP-μ-1, AP-σ-1 complex with inositole hexakisphosphate</scene>.
*<scene name='55/559108/Cv/6'>Inositole hexakisphosphate binding site</scene> ([[2vgl]]).<ref name="Ad">PMID:12086608</ref>


'''AP-α'''
The '''AP-1 complex''' heterotetramer μ subunit packages insulin receptor substrates into clathrin-coated vescicles.  This is needed for insulin/insulin-like growth factor bioactivities including mitogenesis<ref>PMID:23478262</ref>.


[[1b9x]], [[1qts]] – mAP appendage domain – mouse<br />
The '''AP-3 complex''' heterotetramer is involved in the biogenesis of lysosome-related organelles<ref>PMID:15469849</ref>.  It contains subunits β3, μ3, δ and σ3<ref>PMID:9151686</ref>.
[[1qtp]] – mAP appendage domain (mutant)<br />
[[1ky6]], [[1kyd]] – mAP appendage domain + epsin peptide<br />
[[1ky7]] – mAP appendage domain + amphiphysin peptide<br />
[[1kyf]], [[1kyu]] – mAP appendage domain + eps15 peptide<br />
[[1w80]], [[2vj0]] – mAP appendage domain + synaptojanin peptide<br />
[[3hs8]] – mAP appendage domain + intersectin peptide<br />


'''AP-β'''
The '''AP-4 complex''' heterotetramer is involved in the targeting proteins from the trans-Golgi network (TGN) to yhr endosomal-lysosomal system.  It contains subunits β-type, μ-type, ε and σ-type<ref>PMID:11409905</ref>.


[[1e42]] - hAP appendage domain - human<br />
==3D structures of adaptin==
[[2g30]] – hAP appendage domain + ARH peptide<br />
[[Adaptin 3D structures]]
[[2iv9]] – hAP appendage domain + eps15 peptide<br />
[[2iv8]] – hAP appendage domain + B-arrestin peptide<br />
[[3h1z]] – hAP appendage domain + PTDINSPKI-γ peptide<br />
[[3hs9]] – hAP appendage domain + intersectin peptide<br />


'''AP-γ'''
</StructureSection>


[[1iu1]], [[2e9g]], [[4bcx]] – hAP ear domain<br />
[[2ymt]] – hAP ear domain + peptide<br />
[[3zhf]] – hAP ear domain + large envelope protein peptide<br />
[[1gyu]], [[2a7b]] – mAP appendage domain <br />
[[1gyv]], [[1gyw]], [[3zy7]] – mAP appendage domain (mutant)<br />


'''AP-δ'''
== References ==
 
<references/>
[[4afi]] – hAP/vesicle-associated membrane protein 7<br />
[[Category:Topic Page]]
 
'''AP-μ'''
 
[[1bw8]], [[1i31]] – rAP + EGFR peptide<br />
[[1bxx]] – rAP + TGN38 peptide<br />
[[1hes]] – rAP + selectin peptide<br />
[[2bp5]] – rAP + purinoceptor peptide<br />
[[2pr9]] – rAP + GABA receptor peptide<br />
[[3h85]] – rAP appendage domain + PTDINSPKIgamma peptide<br />
[[4emz]], [[4en2]] – mAP sorting motif recognition domain + nef + MHC-I<br />
 
'''AP-α + AP-β + AP-μ + AP-σ'''
 
[[2vgl]] – rAPα + APbeta + APmu + APsigma <br />
[[2jkr]], [[2jkt]] – mAPα + APbeta + APmu + APsigma + CD4 peptide<br />
[[2xa7]] – rAPα + APbeta + APmu + APsigma + cargo peptide<br />
 
'''AP-γ + AP-β + AP-μ + AP-σ'''
 
[[1w63]] – mAPγ + APbeta + APmu + APsigma <br />
[[4hmy]] – mAPγ + APbeta + APmu + APsigma + ADP-ribosylation factor<br />

Latest revision as of 08:28, 28 April 2020

Structure of Adaptin core: AP-α-2 (cyan), AP-β-1 (magenta), AP-μ-1 (green), AP-σ-1 (olive) complex with inositole hexakisphosphate (PDB entry 2vgl)

Drag the structure with the mouse to rotate


References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

Retrieved from "https://proteopedia.org/index.php?title=Adaptin&oldid=3199336"