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{{STRUCTURE_4kvx|  PDB=4kvx  |  SCENE=  }}
===Crystal structure of Naa10 (Ard1) bound to AcCoA===
{{ABSTRACT_PUBMED_23912279}}


==Function==
==Crystal structure of Naa10 (Ard1) bound to AcCoA==
[[http://www.uniprot.org/uniprot/ARD1_SCHPO ARD1_SCHPO]] Catalytic component of the NatA N-terminal acetyltransferase, which catalyzes acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover (By similarity).  
<StructureSection load='4kvx' size='340' side='right'caption='[[4kvx]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4kvx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KVX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kvx OCA], [https://pdbe.org/4kvx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kvx RCSB], [https://www.ebi.ac.uk/pdbsum/4kvx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kvx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARD1_SCHPO ARD1_SCHPO] Catalytic component of the NatA N-terminal acetyltransferase, which catalyzes acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
N-terminal acetylation is ubiquitous among eukaryotic proteins and controls a myriad of biological processes. Of the N-terminal acetyltransferases (NATs) that facilitate this cotranslational modification, the heterodimeric NatA complex has the most diversity for substrate selection and modifies the majority of all N-terminally acetylated proteins. Here, we report the X-ray crystal structure of the 100-kDa holo-NatA complex from Schizosaccharomyces pombe, in the absence and presence of a bisubstrate peptide-CoA-conjugate inhibitor, as well as the structure of the uncomplexed Naa10p catalytic subunit. The NatA-Naa15p auxiliary subunit contains 13 tetratricopeptide motifs and adopts a ring-like topology that wraps around the NatA-Naa10p subunit, an interaction that alters the Naa10p active site for substrate-specific acetylation. These studies have implications for understanding the mechanistic details of other NAT complexes and how regulatory subunits modulate the activity of the broader family of protein acetyltransferases.


==About this Structure==
Molecular basis for N-terminal acetylation by the heterodimeric NatA complex.,Liszczak G, Goldberg JM, Foyn H, Petersson EJ, Arnesen T, Marmorstein R Nat Struct Mol Biol. 2013 Aug 4. doi: 10.1038/nsmb.2636. PMID:23912279<ref>PMID:23912279</ref>
[[4kvx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KVX OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023912279</ref><references group="xtra"/><references/>
</div>
[[Category: Peptide alpha-N-acetyltransferase]]
<div class="pdbe-citations 4kvx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe 972h-]]
[[Category: Schizosaccharomyces pombe 972h-]]
[[Category: Liszczak, G P.]]
[[Category: Liszczak GP]]
[[Category: Marmorstein, R Q.]]
[[Category: Marmorstein RQ]]
[[Category: Acetyltransferase]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/4kvx"