4c3i: Difference between revisions

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New page: '''Unreleased structure''' The entry 4c3i is ON HOLD Authors: Legrand, P. Description: Multiprotein complex
 
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'''Unreleased structure'''


The entry 4c3i is ON HOLD
==Structure of 14-subunit RNA polymerase I at 3.0 A resolution, crystal form C2-100==
<StructureSection load='4c3i' size='340' side='right'caption='[[4c3i]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4c3i]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C3I FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c3i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3i OCA], [https://pdbe.org/4c3i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c3i RCSB], [https://www.ebi.ac.uk/pdbsum/4c3i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c3i ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPAC2_YEAST RPAC2_YEAST] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protein biosynthesis depends on the availability of ribosomes, which in turn relies on ribosomal RNA production. In eukaryotes, this process is carried out by RNA polymerase I (Pol I), a 14-subunit enzyme, the activity of which is a major determinant of cell growth. Here we present the crystal structure of Pol I from Saccharomyces cerevisiae at 3.0 A resolution. The Pol I structure shows a compact core with a wide DNA-binding cleft and a tightly anchored stalk. An extended loop mimics the DNA backbone in the cleft and may be involved in regulating Pol I transcription. Subunit A12.2 extends from the A190 jaw to the active site and inserts a transcription elongation factor TFIIS-like zinc ribbon into the nucleotide triphosphate entry pore, providing insight into the role of A12.2 in RNA cleavage and Pol I insensitivity to alpha-amanitin. The A49-A34.5 heterodimer embraces subunit A135 through extended arms, thereby contacting and potentially regulating subunit A12.2.


Authors: Legrand, P.
Crystal structure of the 14-subunit RNA polymerase I.,Fernandez-Tornero C, Moreno-Morcillo M, Rashid UJ, Taylor NM, Ruiz FM, Gruene T, Legrand P, Steuerwald U, Muller CW Nature. 2013 Oct 31;502(7473):644-9. doi: 10.1038/nature12636. Epub 2013 Oct 23. PMID:24153184<ref>PMID:24153184</ref>


Description: Multiprotein complex
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4c3i" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Fernandez-Tornero C]]
[[Category: Gruene T]]
[[Category: Legrand P]]
[[Category: Moreno-Morcillo M]]
[[Category: Muller CW]]
[[Category: Rashid UJ]]
[[Category: Ruiz FM]]
[[Category: Steuerwald U]]
[[Category: Taylor NMI]]
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