4mgn: Difference between revisions
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==Co-crystal structure of the G. kaustophilus glyQS T box riboswitch Stem I in complex with tRNA== | |||
<StructureSection load='4mgn' size='340' side='right'caption='[[4mgn]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4mgn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus Geobacillus kaustophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MGN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mgn OCA], [https://pdbe.org/4mgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mgn RCSB], [https://www.ebi.ac.uk/pdbsum/4mgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mgn ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
T box riboswitches are cis-acting RNA elements that bind to tRNA and sense its aminoacylation state to influence gene expression. Here, we present the 3.2 A resolution X-ray crystal structures of the T box Stem I-tRNA complex and tRNA, in isolation. T box Stem I forms an arched conformation with extensive intermolecular contacts to two key points of tRNA, the anticodon and D/T-loops. Free and complexed tRNA exist in significantly different conformations, with the contacts stabilizing flexible D/T-loops and a rearrangement of the D-loop. Using a designed T box RNA/tRNA system, we demonstrate that the T box riboswitch monitors the length and orientation of two essential contacts. Length or orientation mismatches engineered into the T box riboswitch and tRNA disrupt the complex, whereas simultaneous insertion of full helical turns realigns the interfaces and restores interaction between artificially elongated T box riboswitch and tRNA molecules. | |||
Structural Determinants for Geometry and Information Decoding of tRNA by T Box Leader RNA.,Grigg JC, Ke A Structure. 2013 Oct 1. pii: S0969-2126(13)00346-8. doi:, 10.1016/j.str.2013.09.001. PMID:24095061<ref>PMID:24095061</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4mgn" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Riboswitch 3D structures|Riboswitch 3D structures]] | |||
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Geobacillus kaustophilus]] | |||
[[Category: Large Structures]] | |||
[[Category: Grigg JC]] | |||
[[Category: Ke A]] | |||
Latest revision as of 16:37, 20 September 2023
Co-crystal structure of the G. kaustophilus glyQS T box riboswitch Stem I in complex with tRNA
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