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{{STRUCTURE_3was|  PDB=3was  |  SCENE=  }}
===Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with Man-Glc+PO4===
{{ABSTRACT_PUBMED_23954514}}


==Function==
==Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with Man-Glc+PO4==
[[http://www.uniprot.org/uniprot/MGP_BACFN MGP_BACFN]] Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose. Involved in a mannan catabolic pathway which feeds into glycolysis.<ref>PMID:21539815</ref>
<StructureSection load='3was' size='340' side='right'caption='[[3was]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3was]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_fragilis_NCTC_9343 Bacteroides fragilis NCTC 9343]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WAS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3was FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3was OCA], [https://pdbe.org/3was PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3was RCSB], [https://www.ebi.ac.uk/pdbsum/3was PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3was ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MGP_BACFN MGP_BACFN] Converts 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc) to mannose 1-phosphate (Man1P) and glucose. Involved in a mannan catabolic pathway which feeds into glycolysis.<ref>PMID:21539815</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of a novel component of the mannan biodegradation system, 4-O-beta-d-mannosyl-d-glucose phosphorylase (MGP), was determined to a 1.68-A resolution. The structure of the enzyme revealed a unique homohexameric structure, which was formed by using two helices attached to the N-terminus and C-terminus as a tab for sticking between subunits. The structures of MGP complexes with genuine substrates, 4-O-beta-d-mannosyl-d-glucose and phosphate, and the product d-mannose-1-phosphate were also determined. The complex structures revealed that the invariant residue Asp131, which is supposed to be the general acid/base, did not exist close to the glycosidic Glc-O4 atom, which should be protonated in the catalytic reaction. Also, no solvent molecule that might mediate a proton transfer from Asp131 was observed in the substrate complex structure, suggesting that the catalytic mechanism of MGP is different from those of known disaccharide phosphorylases.


==About this Structure==
Structure of Novel Enzyme in Mannan Biodegradation Process 4-O-beta-d-Mannosyl-d-Glucose Phosphorylase MGP.,Nakae S, Ito S, Higa M, Senoura T, Wasaki J, Hijikata A, Shionyu M, Ito S, Shirai T J Mol Biol. 2013 Aug 14. pii: S0022-2836(13)00505-6. doi:, 10.1016/j.jmb.2013.08.002. PMID:23954514<ref>PMID:23954514</ref>
[[3was]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteroides_fragilis_nctc_9343 Bacteroides fragilis nctc 9343]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WAS OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023954514</ref><references group="xtra"/><references/>
</div>
[[Category: 4-O-beta-D-mannosyl-D-glucose phosphorylase]]
<div class="pdbe-citations 3was" style="background-color:#fffaf0;"></div>
[[Category: Bacteroides fragilis nctc 9343]]
== References ==
[[Category: Higa, M.]]
<references/>
[[Category: Hijikata, A.]]
__TOC__
[[Category: Ito, S.]]
</StructureSection>
[[Category: Nakae, S.]]
[[Category: Bacteroides fragilis NCTC 9343]]
[[Category: Senoura, T.]]
[[Category: Large Structures]]
[[Category: Shionyu, M.]]
[[Category: Higa M]]
[[Category: Shirai, T.]]
[[Category: Hijikata A]]
[[Category: Wasaki, J.]]
[[Category: Ito S]]
[[Category: 5-bladed beta propeller fold]]
[[Category: Nakae S]]
[[Category: Mannan biodegradation]]
[[Category: Senoura T]]
[[Category: Phosphorylase]]
[[Category: Shionyu M]]
[[Category: Transferase]]
[[Category: Shirai T]]
[[Category: Wasaki J]]

Latest revision as of 13:02, 8 November 2023

Crystal structure of 4-O-beta-D-mannosyl-D-glucose phosphorylase MGP complexed with Man-Glc+PO4

3was, resolution 1.50Å

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