2o3m: Difference between revisions
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== | ==Monomeric G-DNA tetraplex from human C-kit promoter== | ||
<StructureSection load='2o3m' size='340' side='right'caption='[[2o3m]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2o3m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O3M FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o3m OCA], [https://pdbe.org/2o3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o3m RCSB], [https://www.ebi.ac.uk/pdbsum/2o3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o3m ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The c-kit oncogene is an important target in the treatment of gastrointestinal tumors. A potential approach to inhibition of the expression of this gene involves selective stabilization of G-quadruplex structures that may be induced to form in the c-kit promoter region. Here we report on the structure of an unprecedented intramolecular G-quadruplex formed by a G-rich sequence in the c-kit promoter in K+ solution. The structure represents a new folding topology with several unique features. Most strikingly, an isolated guanine is involved in G-tetrad core formation, despite the presence of four three-guanine tracts. There are four loops: two single-residue double-chain-reversal loops, a two-residue loop, and a five-residue stem-loop, which contain base-pairing alignments. This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to the promoter DNA of c-kit. | The c-kit oncogene is an important target in the treatment of gastrointestinal tumors. A potential approach to inhibition of the expression of this gene involves selective stabilization of G-quadruplex structures that may be induced to form in the c-kit promoter region. Here we report on the structure of an unprecedented intramolecular G-quadruplex formed by a G-rich sequence in the c-kit promoter in K+ solution. The structure represents a new folding topology with several unique features. Most strikingly, an isolated guanine is involved in G-tetrad core formation, despite the presence of four three-guanine tracts. There are four loops: two single-residue double-chain-reversal loops, a two-residue loop, and a five-residue stem-loop, which contain base-pairing alignments. This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to the promoter DNA of c-kit. | ||
Structure of an unprecedented G-quadruplex scaffold in the human c-kit promoter.,Phan AT, Kuryavyi V, Burge S, Neidle S, Patel DJ J Am Chem Soc. 2007 Apr 11;129(14):4386-92. Epub 2007 Mar 16. PMID:17362008<ref>PMID:17362008</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2o3m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Burge | [[Category: Burge S]] | ||
[[Category: Kuryavyi | [[Category: Kuryavyi VV]] | ||
[[Category: Neidle | [[Category: Neidle S]] | ||
[[Category: Patel | [[Category: Patel DJ]] | ||
[[Category: Phan | [[Category: Phan AT]] | ||
Latest revision as of 12:52, 20 December 2023
Monomeric G-DNA tetraplex from human C-kit promoter
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