2oa1: Difference between revisions

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[[Image:2oa1.gif|left|200px]]<br /><applet load="2oa1" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2oa1, resolution 2.150&Aring;" />
'''Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the L-Tryptophan with FAD complex'''<br />


==About this Structure==
==Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the L-Tryptophan with FAD complex==
2OA1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=TRP:'>TRP</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=ADN:'>ADN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Cl+Binding+Site+For+Residue+A+2001'>AC1</scene>, <scene name='pdbsite=AC2:TRP+Binding+Site+For+Residue+A+2002'>AC2</scene>, <scene name='pdbsite=AC3:TRP+Binding+Site+For+Residue+A+2003'>AC3</scene>, <scene name='pdbsite=AC4:Fad+Binding+Site+For+Residue+A+2004'>AC4</scene> and <scene name='pdbsite=AC5:Adn+Binding+Site+For+Residue+B+2005'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA1 OCA].  
<StructureSection load='2oa1' size='340' side='right'caption='[[2oa1]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
 
== Structural highlights ==
==Reference==
<table><tr><td colspan='2'>[[2oa1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OA1 FirstGlance]. <br>
The structure of flavin-dependent tryptophan 7-halogenase RebH., Bitto E, Huang Y, Bingman CA, Singh S, Thorson JS, Phillips GN Jr, Proteins. 2008 Jan 1;70(1):289-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17876823 17876823]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
[[Category: Lechevalieria aerocolonigenes]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
[[Category: Single protein]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oa1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oa1 OCA], [https://pdbe.org/2oa1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oa1 RCSB], [https://www.ebi.ac.uk/pdbsum/2oa1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oa1 ProSAT]</span></td></tr>
[[Category: Bingman, C A.]]
</table>
[[Category: Bitto, E.]]
== Function ==
[[Category: Jr., G N.Phillips.]]
[https://www.uniprot.org/uniprot/REBH_LENAE REBH_LENAE] Involved in the biosynthesis of the indolocarbazole antitumor agent rebeccamycin. Catalyzes the chlorination of tryptophan (Trp) at C7 position to yield 7-chlorotryptophan. The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:11983340</ref> <ref>PMID:15743914</ref> <ref>PMID:17260957</ref>
[[Category: Singh, S.]]
== Evolutionary Conservation ==
[[Category: ADN]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: CL]]
Check<jmol>
[[Category: FAD]]
  <jmolCheckbox>
[[Category: TRP]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/2oa1_consurf.spt"</scriptWhenChecked>
[[Category: biosynthetic protein]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: flavin]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: flavoprotein]]
  </jmolCheckbox>
[[Category: halogenase]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oa1 ConSurf].
[[Category: rebeccamycin]]
<div style="clear:both"></div>
[[Category: rebh]]
== References ==
 
<references/>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:16:18 2008''
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lentzea aerocolonigenes]]
[[Category: Bingman CA]]
[[Category: Bitto E]]
[[Category: Phillips Jr GN]]
[[Category: Singh S]]
Retrieved from "https://proteopedia.org/2oa1"