4lku: Difference between revisions

Latest revision as of 12:20, 1 March 2024

Structure of the C-terminal domain of the E. coli mechanosensitive channel of large conductance

Structural highlights

4lku is a 5 chain structure with sequence from Escherichia coli DH1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.45Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MSCL_ECOLI Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. Participates in the regulation of osmotic pressure changes within the cell. Opens at a pressure just below that which would cause cell disruption and death. The force required to trigger channel opening depends on the membrane lipids composition.^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 1999 Apr 1;18(7):1730-7. PMID:10202137 doi:https://dx.doi.org/10.1093/emboj/18.7.1730
↑ Yang LM, Zhong D, Blount P. Chimeras reveal a single lipid-interface residue that controls MscL channel kinetics as well as mechanosensitivity. Cell Rep. 2013 Feb 21;3(2):520-7. doi: 10.1016/j.celrep.2013.01.018. Epub 2013 , Feb 14. PMID:23416054 doi:https://dx.doi.org/10.1016/j.celrep.2013.01.018
↑ Zhong D, Blount P. Phosphatidylinositol is crucial for the mechanosensitivity of Mycobacterium tuberculosis MscL. Biochemistry. 2013 Aug 13;52(32):5415-20. doi: 10.1021/bi400790j. Epub 2013 Aug , 1. PMID:23875651 doi:https://dx.doi.org/10.1021/bi400790j
↑ Sukharev SI, Blount P, Martinac B, Blattner FR, Kung C. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature. 1994 Mar 17;368(6468):265-8. PMID:7511799 doi:https://dx.doi.org/10.1038/368265a0
↑ Moe PC, Blount P, Kung C. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol. 1998 May;28(3):583-92. doi: 10.1046/j.1365-2958.1998.00821.x. PMID:9632260 doi:https://dx.doi.org/10.1046/j.1365-2958.1998.00821.x

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OCA

[1] Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. EMBO J. 1999 Apr 1;18(7):1730-7. PMID:10202137 doi:https://dx.doi.org/10.1093/emboj/18.7.1730

[2] Yang LM, Zhong D, Blount P. Chimeras reveal a single lipid-interface residue that controls MscL channel kinetics as well as mechanosensitivity. Cell Rep. 2013 Feb 21;3(2):520-7. doi: 10.1016/j.celrep.2013.01.018. Epub 2013 , Feb 14. PMID:23416054 doi:https://dx.doi.org/10.1016/j.celrep.2013.01.018

[3] Zhong D, Blount P. Phosphatidylinositol is crucial for the mechanosensitivity of Mycobacterium tuberculosis MscL. Biochemistry. 2013 Aug 13;52(32):5415-20. doi: 10.1021/bi400790j. Epub 2013 Aug , 1. PMID:23875651 doi:https://dx.doi.org/10.1021/bi400790j

[4] Sukharev SI, Blount P, Martinac B, Blattner FR, Kung C. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Nature. 1994 Mar 17;368(6468):265-8. PMID:7511799 doi:https://dx.doi.org/10.1038/368265a0

[5] Moe PC, Blount P, Kung C. Functional and structural conservation in the mechanosensitive channel MscL implicates elements crucial for mechanosensation. Mol Microbiol. 1998 May;28(3):583-92. doi: 10.1046/j.1365-2958.1998.00821.x. PMID:9632260 doi:https://dx.doi.org/10.1046/j.1365-2958.1998.00821.x

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-{{STRUCTURE_4lku|  PDB=4lku  |  SCENE=  }}
-===Structure of the C-terminal domain of the E. coli mechanosensitive channel of large conductance===
-{{ABSTRACT_PUBMED_24038743}}
-==Function==
+==Structure of the C-terminal domain of the E. coli mechanosensitive channel of large conductance==
-[[http://www.uniprot.org/uniprot/C9QXJ2_ECOD1 C9QXJ2_ECOD1]] Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell (By similarity).[SAAS:SAAS001185_004_016395][HAMAP-Rule:MF_00115]
+<StructureSection load='4lku' size='340' side='right'caption='[[4lku]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4lku]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH1 Escherichia coli DH1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LKU FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lku OCA], [https://pdbe.org/4lku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lku RCSB], [https://www.ebi.ac.uk/pdbsum/4lku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lku ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MSCL_ECOLI MSCL_ECOLI] Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Forms a nonselective ion channel with a conductance of about 4 nanosiemens. Participates in the regulation of osmotic pressure changes within the cell. Opens at a pressure just below that which would cause cell disruption and death. The force required to trigger channel opening depends on the membrane lipids composition.<ref>PMID:10202137</ref> <ref>PMID:23416054</ref> <ref>PMID:23875651</ref> <ref>PMID:7511799</ref> <ref>PMID:9632260</ref>
-==About this Structure==
+==See Also==
-[[4lku]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LKU OCA].
+*[[Ion channels 3D structures|Ion channels 3D structures]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:024038743</ref><references group="xtra"/><references/>
+__TOC__
-[[Category: Rees, D C.]]
+</StructureSection>
-[[Category: Walton, T A.]]
+[[Category: Escherichia coli DH1]]
-[[Category: Pentamer]]
+[[Category: Large Structures]]
-[[Category: Transport protein]]
+[[Category: Rees DC]]
+[[Category: Walton TA]]

4lku: Difference between revisions

Latest revision as of 12:20, 1 March 2024

Structure of the C-terminal domain of the E. coli mechanosensitive channel of large conductance

Structural highlights

Function

See Also

References

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