4mzz: Difference between revisions
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New page: '''Unreleased structure''' The entry 4mzz is ON HOLD Authors: Malashkevich, V.N., Dowd, T.L., Almo, S.C., New York Structural Genomics Research Consortium (NYSGRC) Description: Crystal... |
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The | ==Crystal structure of Bovine 3 Glu-Osteocalcin.== | ||
<StructureSection load='4mzz' size='340' side='right'caption='[[4mzz]], [[Resolution|resolution]] 1.88Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4mzz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MZZ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mzz OCA], [https://pdbe.org/4mzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mzz RCSB], [https://www.ebi.ac.uk/pdbsum/4mzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mzz ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/OSTCN_BOVIN OSTCN_BOVIN] Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The 3 Glu form of osteocalcin (3 Glu-OCN) is increased in serum during low vitamin K intake or oral anticoagulant use (warfarin). Previous reports using circular dichroism show it is less structured than 3 Gla Ca(2)(+)-osteocalcin and does not bind strongly to bone mineral. Recent studies have suggested a role for 3 Glu-OCN as a potential regulator of glucose metabolism. A G-protein-coupled receptor, GPRC6a, found in the pancreas and testes was identified as the putative osteocalcin receptor. The purpose of this study is to determine the high-resolution structure of bovine 3 Glu-OCN, using X-ray crystallography, to understand molecular interactions with mineral and the GPRC6a receptor. Diffraction quality crystals of thermally decarboxylated bovine osteocalcin were grown, and the crystal structure was determined to 1.88 A resolution. The final refined structure contained residues 17-47 and, like 3 Gla Ca(2)(+)-OCN, consisted of three alpha-helices surrounding a hydrophobic core, a C23-C29 disulfide bond between two of the helices, and no bound Ca(2)(+). Thus, the helical structure of 3 Glu-OCN is Ca(2)(+)-independent but similar to that of 3 Gla Ca(2)(+)-OCN. A reduced level of mineral binding could result from a lower number of Ca(2)(+) coordinating ligands on 3 Glu-OCN. The structure suggests the GPRC6a receptor may respond to helical osteocalcin and will aid in providing molecular mechanistic insight into the role of 3 Glu-OCN in glucose homeostasis. | |||
X-ray crystal structure of bovine 3 Glu-osteocalcin.,Malashkevich VN, Almo SC, Dowd TL Biochemistry. 2013 Nov 26;52(47):8387-92. doi: 10.1021/bi4010254. Epub 2013 Nov, 12. PMID:24138653<ref>PMID:24138653</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4mzz" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bos taurus]] | |||
[[Category: Large Structures]] | |||
[[Category: Almo SC]] | |||
[[Category: Dowd TL]] | |||
[[Category: Malashkevich VN]] | |||
