1w6s: Difference between revisions

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[[Image:1w6s.gif|left|200px]]<br />
<applet load="1w6s" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1w6s, resolution 1.2&Aring;" />
'''THE HIGH RESOLUTION STRUCTURE OF METHANOL DEHYDROGENASE FROM METHYLOBACTERIUM EXTORQUENS'''<br />


==Overview==
==The high resolution structure of methanol dehydrogenase from methylobacterium extorquens==
The crystal structure of methanol dehydrogenase (MDH) from, Methylobacterium extorquens has been refined without stereochemical, restraints at a resolution of 1.2 A. The high-resolution data have defined, the conformation of the tricyclic pyrroloquinoline quinone (PQQ) cofactor, ring as entirely planar. The detailed definition of the active-site, geometry has shown many features that are similar to the, quinohaemo-protein alcohol dehydrogenases from Comamonas testosteroni and, Pseudomonas putida, both of which possess MDH-like and cytochrome c-like, domains. Conserved features between the two types of PQQ-containing enzyme, suggest a common pathway for electron transfer between MDH and its, physiological electron acceptor cytochrome cL. A pathway for proton, transfer from the active site ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15608378 (full description)]]
<StructureSection load='1w6s' size='340' side='right'caption='[[1w6s]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1w6s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W6S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w6s OCA], [https://pdbe.org/1w6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w6s RCSB], [https://www.ebi.ac.uk/pdbsum/1w6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w6s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DHM1_METEA DHM1_METEA] Catalyzes the oxidation of primary alcohols including methanol.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w6/1w6s_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w6s ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of methanol dehydrogenase (MDH) from Methylobacterium extorquens has been refined without stereochemical restraints at a resolution of 1.2 A. The high-resolution data have defined the conformation of the tricyclic pyrroloquinoline quinone (PQQ) cofactor ring as entirely planar. The detailed definition of the active-site geometry has shown many features that are similar to the quinohaemo-protein alcohol dehydrogenases from Comamonas testosteroni and Pseudomonas putida, both of which possess MDH-like and cytochrome c-like domains. Conserved features between the two types of PQQ-containing enzyme suggest a common pathway for electron transfer between MDH and its physiological electron acceptor cytochrome cL. A pathway for proton transfer from the active site to the bulk solvent is also suggested.


==About this Structure==
The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens.,Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):75-9. Epub 2004 Dec, 17. PMID:15608378<ref>PMID:15608378</ref>
1W6S is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]] with CA, PQQ and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W6S OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens., Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB, Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):75-9. Epub 2004 Dec, 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15608378 15608378]
</div>
[[Category: Alcohol dehydrogenase (acceptor)]]
<div class="pdbe-citations 1w6s" style="background-color:#fffaf0;"></div>
[[Category: Methylobacterium extorquens]]
[[Category: Protein complex]]
[[Category: Anthony, C.]]
[[Category: Coates, L.]]
[[Category: Cooper, J.B.]]
[[Category: Erskine, P.T.]]
[[Category: Gill, R.]]
[[Category: Mohammed, F.]]
[[Category: Williams, P.A.]]
[[Category: Wood, S.P.]]
[[Category: CA]]
[[Category: GOL]]
[[Category: PQQ]]
[[Category: anisotropic]]
[[Category: calcium-binding]]
[[Category: electron transfer]]
[[Category: methanol utilization]]
[[Category: oxidoreductase]]
[[Category: pqq]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:26:52 2007''
==See Also==
*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
*[[Methanol dehydrogenase|Methanol dehydrogenase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methylorubrum extorquens]]
[[Category: Anthony C]]
[[Category: Coates L]]
[[Category: Cooper JB]]
[[Category: Erskine PT]]
[[Category: Gill R]]
[[Category: Mohammed F]]
[[Category: Williams PA]]
[[Category: Wood SP]]
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