4n1d: Difference between revisions

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~~'''Unreleased structure'''~~

The entry ~~4n1d~~ is ~~ON HOLD~~

==Nodal/BMP2 chimera NB250==

== Structural highlights ==

<table><tr><td colspan='2'>[[4n1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N1D FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.912Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n1d OCA], [https://pdbe.org/4n1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n1d RCSB], [https://www.ebi.ac.uk/pdbsum/4n1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n1d ProSAT]</span></td></tr>

</table>

== Disease ==

[https://www.uniprot.org/uniprot/NODAL_HUMAN NODAL_HUMAN] Alobar holoprosencephaly;Situs ambiguus;Lobar holoprosencephaly;Semilobar holoprosencephaly;Midline interhemispheric variant of holoprosencephaly;Septopreoptic holoprosencephaly;Situs inversus totalis;Microform holoprosencephaly. The disease is caused by variants affecting the gene represented in this entry.

== Function ==

[https://www.uniprot.org/uniprot/NODAL_HUMAN NODAL_HUMAN] Essential for mesoderm formation and axial patterning during embryonic development.[https://www.uniprot.org/uniprot/BMP2_HUMAN BMP2_HUMAN] Induces cartilage and bone formation.

== Publication Abstract from PubMed ==

Nodal, a member of the TGF-beta superfamily, plays an important role in vertebrate and invertebrate early development. The biochemical study of Nodal and its signaling pathway has been a challenge, mainly because of difficulties in producing the protein in sufficient quantities. We have developed a library of stable, chemically refoldable Nodal/BMP2 chimeric ligands (NB2 library). Three chimeras, named NB250, NB260, and NB264, show Nodal-like signaling properties including dependence on the co-receptor Cripto and activation of the Smad2 pathway. NB250, like Nodal, alters heart looping during the establishment of embryonic left-right asymmetry, and both NB250 and NB260, as well as Nodal, induce chondrogenic differentiation of human adipose-derived stem cells. This Nodal-induced differentiation is shown to be more efficient than BPM2-induced differentiation. Interestingly, the crystal structure of NB250 shows a backbone scaffold similar to that of BMP2. Our results show that these chimeric ligands may have therapeutic implications in cartilage injuries.

~~Authors~~: ~~Esquivies~~, L

Designer Nodal/BMP2 Chimeras Mimic Nodal Signaling, Promote Chondrogenesis, and Reveal a BMP2-like Structure.,Esquivies L, Blackler A, Peran M, Rodriguez-Esteban C, Izpisua Belmonte JC, Booker E, Gray PC, Ahn C, Kwiatkowski W, Choe S J Biol Chem. 2014 Jan 17;289(3):1788-97. doi: 10.1074/jbc.M113.529180. Epub 2013 , Dec 5. PMID:24311780<ref>PMID:24311780</ref>

~~Description~~: ~~Nodal~~/~~BMP2 chimera NB250~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

==See Also==

*[[Bone morphogenetic protein 3D structures|Bone morphogenetic protein 3D structures]]

== References ==

__TOC__

</StructureSection>

[[Category: Homo sapiens]]

[[Category: Large Structures]]

[[Category: Esquivies L]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4n1d is ON HOLD
+==Nodal/BMP2 chimera NB250==
+<StructureSection load='4n1d' size='340' side='right'caption='[[4n1d]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4n1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N1D FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.912&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n1d OCA], [https://pdbe.org/4n1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n1d RCSB], [https://www.ebi.ac.uk/pdbsum/4n1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n1d ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/NODAL_HUMAN NODAL_HUMAN] Alobar holoprosencephaly;Situs ambiguus;Lobar holoprosencephaly;Semilobar holoprosencephaly;Midline interhemispheric variant of holoprosencephaly;Septopreoptic holoprosencephaly;Situs inversus totalis;Microform holoprosencephaly. The disease is caused by variants affecting the gene represented in this entry.
+== Function ==
+[https://www.uniprot.org/uniprot/NODAL_HUMAN NODAL_HUMAN] Essential for mesoderm formation and axial patterning during embryonic development.[https://www.uniprot.org/uniprot/BMP2_HUMAN BMP2_HUMAN] Induces cartilage and bone formation.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Nodal, a member of the TGF-beta superfamily, plays an important role in vertebrate and invertebrate early development. The biochemical study of Nodal and its signaling pathway has been a challenge, mainly because of difficulties in producing the protein in sufficient quantities. We have developed a library of stable, chemically refoldable Nodal/BMP2 chimeric ligands (NB2 library). Three chimeras, named NB250, NB260, and NB264, show Nodal-like signaling properties including dependence on the co-receptor Cripto and activation of the Smad2 pathway. NB250, like Nodal, alters heart looping during the establishment of embryonic left-right asymmetry, and both NB250 and NB260, as well as Nodal, induce chondrogenic differentiation of human adipose-derived stem cells. This Nodal-induced differentiation is shown to be more efficient than BPM2-induced differentiation. Interestingly, the crystal structure of NB250 shows a backbone scaffold similar to that of BMP2. Our results show that these chimeric ligands may have therapeutic implications in cartilage injuries.
-Authors: Esquivies, L
+Designer Nodal/BMP2 Chimeras Mimic Nodal Signaling, Promote Chondrogenesis, and Reveal a BMP2-like Structure.,Esquivies L, Blackler A, Peran M, Rodriguez-Esteban C, Izpisua Belmonte JC, Booker E, Gray PC, Ahn C, Kwiatkowski W, Choe S J Biol Chem. 2014 Jan 17;289(3):1788-97. doi: 10.1074/jbc.M113.529180. Epub 2013 , Dec 5. PMID:24311780<ref>PMID:24311780</ref>
-Description: Nodal/BMP2 chimera NB250
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4n1d" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Bone morphogenetic protein 3D structures|Bone morphogenetic protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Esquivies L]]