|
|
| (10 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:2ow8.gif|left|200px]]<br /><applet load="2ow8" size="350" color="white" frame="true" align="right" spinBox="true" | | #REDIRECT [[4v4i]] This PDB entry is obsolete and replaced by 4v4i |
| caption="2ow8, resolution 3.710Å" />
| |
| '''Crystal Structure of a 70S Ribosome-tRNA Complex Reveals Functional Interactions and Rearrangements. This file, 2OW8, contains the 30S ribosome subunit, two tRNA, and mRNA molecules. 50S ribosome subunit is in the file 1VSA.'''<br />
| |
| | |
| ==Overview==
| |
| Our understanding of the mechanism of protein synthesis has undergone rapid progress in recent years as a result of low-resolution X-ray and cryo-EM structures of ribosome functional complexes and high-resolution structures of ribosomal subunits and vacant ribosomes. Here, we present the crystal structure of the Thermus thermophilus 70S ribosome containing a model mRNA and two tRNAs at 3.7 A resolution. Many structural details of the interactions between the ribosome, tRNA, and mRNA in the P and E sites and the ways in which tRNA structure is distorted by its interactions with the ribosome are seen. Differences between the conformations of vacant and tRNA-bound 70S ribosomes suggest an induced fit of the ribosome structure in response to tRNA binding, including significant changes in the peptidyl-transferase catalytic site.
| |
| | |
| ==About this Structure==
| |
| 2OW8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. This structure supersedes the now removed PDB entry 2I1C. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OW8 OCA].
| |
| | |
| ==Reference==
| |
| Crystal structure of a 70S ribosome-tRNA complex reveals functional interactions and rearrangements., Korostelev A, Trakhanov S, Laurberg M, Noller HF, Cell. 2006 Sep 22;126(6):1065-77. Epub 2006 Sep 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16962654 16962654]
| |
| [[Category: Escherichia coli]]
| |
| [[Category: Protein complex]]
| |
| [[Category: Thermus thermophilus]]
| |
| [[Category: Korostelev, A.]]
| |
| [[Category: Laurberg, M.]]
| |
| [[Category: Noller, H F.]]
| |
| [[Category: Trakhanov, S.]]
| |
| [[Category: crystal structure]]
| |
| [[Category: e site]]
| |
| [[Category: functional complex]]
| |
| [[Category: p site]]
| |
| [[Category: peptidyl transferase center]]
| |
| [[Category: ribosome]]
| |
| [[Category: trna]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:23:11 2008''
| |
This PDB entry is obsolete and replaced by 4v4i
