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| [[Image:2p73.jpg|left|200px]]<br /><applet load="2p73" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2p73, resolution 2.300Å" />
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| '''crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1'''<br />
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| ==Overview== | | ==crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1== |
| Paramecium bursaria chlorella virus-1 encodes at least five putative glycosyltransferases that are probably involved in the synthesis of the glycan components of the viral major capsid protein. The 1.6 A crystal structure of one of these glycosyltransferases (A64R) has a mixed alpha/beta fold containing a central, six-stranded beta sheet flanked by alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases which utilize UDP as the sugar carrier. The structure of the complex of A64R, UDP-glucose, and Mn(2+) showed that the largest conformational change occurred when hydrogen bonds were formed with the ligands. Unlike UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the sugar donor for A64R, consistent with glucose occurring in the virus major capsid protein glycans.
| | <StructureSection load='2p73' size='340' side='right'caption='[[2p73]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[2p73]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P73 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P73 FirstGlance]. <br> |
| 2P73 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=UDP:'>UDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P73 OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p73 OCA], [https://pdbe.org/2p73 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p73 RCSB], [https://www.ebi.ac.uk/pdbsum/2p73 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p73 ProSAT]</span></td></tr> |
| Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17850743 17850743]
| | </table> |
| [[Category: Paramecium bursaria chlorella virus 1]]
| | == Function == |
| [[Category: Single protein]]
| | [https://www.uniprot.org/uniprot/Q89399_PBCV1 Q89399_PBCV1] |
| [[Category: Etten, J L.Van.]] | | __TOC__ |
| [[Category: Rossmann, M G.]]
| | </StructureSection> |
| [[Category: Xiang, Y.]]
| | [[Category: Large Structures]] |
| [[Category: Zhang, Y.]] | | [[Category: Paramecium bursaria Chlorella virus 1]] |
| [[Category: MN]] | | [[Category: Rossmann MG]] |
| [[Category: UDP]] | | [[Category: Van Etten JL]] |
| [[Category: crystal structure]] | | [[Category: Xiang Y]] |
| [[Category: glycosyltransferase]] | | [[Category: Zhang Y]] |
| [[Category: pbcv-1 a64r]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:26:44 2008''
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