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[[Image:2pbi.jpg|left|200px]]<br /><applet load="2pbi" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2pbi, resolution 1.950&Aring;" />
'''The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure'''<br />


==Overview==
==The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure==
Regulators of G-protein signaling (RGS) proteins enhance the intrinsic GTPase activity of G protein alpha (Galpha) subunits and are vital for proper signaling kinetics downstream of G protein-coupled receptors (GPCRs). R7 subfamily RGS proteins specifically and obligately dimerize with the atypical G protein beta5 (Gbeta5) subunit through an internal G protein gamma (Ggamma)-subunit-like (GGL) domain. Here we present the 1.95-A crystal structure of the Gbeta5-RGS9 complex, which is essential for normal visual and neuronal signal transduction. This structure reveals a canonical RGS domain that is functionally integrated within a molecular complex that is poised for integration of multiple steps during G-protein activation and deactivation.
<StructureSection load='2pbi' size='340' side='right'caption='[[2pbi]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2pbi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PBI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pbi OCA], [https://pdbe.org/2pbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pbi RCSB], [https://www.ebi.ac.uk/pdbsum/2pbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pbi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RGS9_MOUSE RGS9_MOUSE] Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pb/2pbi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pbi ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2PBI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Residue+D+901'>AC1</scene>, <scene name='pdbsite=AC2:Gol+Binding+Site+For+Residue+B+902'>AC2</scene>, <scene name='pdbsite=AC3:Gol+Binding+Site+For+Residue+D+903'>AC3</scene>, <scene name='pdbsite=AC4:Gol+Binding+Site+For+Residue+A+904'>AC4</scene>, <scene name='pdbsite=AC5:Gol+Binding+Site+For+Residue+A+905'>AC5</scene>, <scene name='pdbsite=AC6:Gol+Binding+Site+For+Residue+A+906'>AC6</scene>, <scene name='pdbsite=AC7:Gol+Binding+Site+For+Residue+C+907'>AC7</scene>, <scene name='pdbsite=AC8:Gol+Binding+Site+For+Residue+D+908'>AC8</scene> and <scene name='pdbsite=AC9:Gol+Binding+Site+For+Residue+B+909'>AC9</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBI OCA].
*[[Regulator of G-protein signaling 3D structures|Regulator of G-protein signaling 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of the multifunctional Gbeta5-RGS9 complex., Cheever ML, Snyder JT, Gershburg S, Siderovski DP, Harden TK, Sondek J, Nat Struct Mol Biol. 2008 Feb;15(2):155-62. Epub 2008 Jan 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18204463 18204463]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Cheever ML]]
[[Category: Cheever, M L.]]
[[Category: Gershburg S]]
[[Category: Gershburg, S.]]
[[Category: Harden TK]]
[[Category: Harden, T K.]]
[[Category: Siderovski DP]]
[[Category: Siderovski, D P.]]
[[Category: Snyder JT]]
[[Category: Snyder, J T.]]
[[Category: Sondek J]]
[[Category: Sondek, J.]]
[[Category: GOL]]
[[Category: beta propeller]]
[[Category: dep domain]]
[[Category: dhex domain]]
[[Category: ggl domain]]
[[Category: helix wrap]]
[[Category: rgs domain]]
[[Category: signaling protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:27:54 2008''
Retrieved from "https://proteopedia.org/2pbi"