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[[Image:2pel.jpg|left|200px]]<br /><applet load="2pel" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2pel, resolution 2.25&Aring;" />
'''PEANUT LECTIN'''<br />


==Overview==
==PEANUT LECTIN==
The structure of the complex of the tetrameric peanut lectin with lactose has been refined to an R-value of 16.4% using 2.25 angstroms resolution X-ray diffraction data. The subunit conformation in the structure is similar to that in other legume lectins except in the loops. It has been shown that in the tertiary structure of legume lectins, the short five-stranded sheet plays a major role in connecting the larger flat six-stranded and curved seven-stranded sheets. Furthermore, the loops that connect the strands at the two ends of the seven-stranded sheet curve toward and interact with each other to produce a second hydrophobic core in addition to the one between the two large sheets. The protein-lactose interactions involve the invariant features observed in other legume lectins in addition to those characteristic of peanut lectin. The "open" quaternary association in peanut lectin is stabilised by hydrophobic, hydrogen-bonded and water-mediated interactions. Contrary to the earlier belief, the structure of peanut lectin demonstrates that the variability in quaternary association in legume lectins, despite all of them having nearly the same tertiary structure, is not necessarily caused by covalently bound carbohydrate. An attempt has been made to provide a structural rationale for this variability, on the basis of buried surface areas during dimerisation. A total of 45 water molecules remain invariant when the hydration shells of the four subunits are compared. A majority of them appear to be involved in stabilising loops.
<StructureSection load='2pel' size='340' side='right'caption='[[2pel]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2pel]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arachis_hypogaea Arachis hypogaea]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pel 1pel]. The April 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Concanavalin A and Circular Permutation''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_4 10.2210/rcsb_pdb/mom_2010_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PEL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene>, <scene name='pdbligand=PRD_900008:alpha-lactose'>PRD_900008</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pel OCA], [https://pdbe.org/2pel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pel RCSB], [https://www.ebi.ac.uk/pdbsum/2pel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pel ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LECG_ARAHY LECG_ARAHY] D-galactose specific lectin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pe/2pel_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pel ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2PEL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arachis_hypogaea Arachis hypogaea] with <scene name='pdbligand=LBT:'>LBT</scene>, <scene name='pdbligand=LAT:'>LAT</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1PEL. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEL OCA].
*[[Agglutinin 3D structures|Agglutinin 3D structures]]
 
*[[Galactose-binding lectin|Galactose-binding lectin]]
==Reference==
__TOC__
Conformation, protein-carbohydrate interactions and a novel subunit association in the refined structure of peanut lectin-lactose complex., Banerjee R, Das K, Ravishankar R, Suguna K, Surolia A, Vijayan M, J Mol Biol. 1996 Jun 7;259(2):281-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8656429 8656429]
</StructureSection>
[[Category: Arachis hypogaea]]
[[Category: Arachis hypogaea]]
[[Category: Single protein]]
[[Category: Concanavalin A and Circular Permutation]]
[[Category: Banerjee, R.]]
[[Category: Large Structures]]
[[Category: Das, K.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Ravishankar, R.]]
[[Category: Banerjee R]]
[[Category: Suguna, K.]]
[[Category: Das K]]
[[Category: Surolia, A.]]
[[Category: Ravishankar R]]
[[Category: Vijayan, M.]]
[[Category: Suguna K]]
[[Category: CA]]
[[Category: Surolia A]]
[[Category: LAT]]
[[Category: Vijayan M]]
[[Category: LBT]]
[[Category: MN]]
[[Category: agglutinin]]
[[Category: carbohydrate specificity]]
[[Category: legume lectin]]
[[Category: open quaternary structure]]
[[Category: protein crystallography]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:28:39 2008''
Retrieved from "https://proteopedia.org/2pel"