3zpv: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{STRUCTURE_3zpv|  PDB=3zpv  |  SCENE=  }}
===Crystal structure of Drosophila Pygo PHD finger in complex with Legless HD1 domain===


==Function==
==Crystal structure of Drosophila Pygo PHD finger in complex with Legless HD1 domain==
[[http://www.uniprot.org/uniprot/BCL9_DROME BCL9_DROME]] Involved in signal transduction through the Wnt pathway. [[http://www.uniprot.org/uniprot/PYGO_DROME PYGO_DROME]] Involved in signal transduction through the Wnt pathway.  
<StructureSection load='3zpv' size='340' side='right'caption='[[3zpv]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3zpv]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZPV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.68&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zpv OCA], [https://pdbe.org/3zpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zpv RCSB], [https://www.ebi.ac.uk/pdbsum/3zpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zpv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PYGO_DROME PYGO_DROME] Involved in signal transduction through the Wnt pathway.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pygo proteins promote Armadillo- and beta-catenin-dependent transcription, by relieving Groucho-dependent repression of Wnt targets. Their PHD fingers bind histone H3 tail methylated at lysine 4, and to the HD1 domain of their Legless/BCL9 cofactors, linking Pygo to Armadillo/beta-catenin. Intriguingly, fly Pygo orthologs exhibit a tryptophan &gt; phenylalanine substitution in their histone pocket-divider which reduces their affinity for histones. Here, we use X-ray crystallography and NMR, to discover a conspicuous groove bordering this phenylalanine in the Drosophila PHD-HD1 complex-a semi-aromatic cage recognizing asymmetrically methylated arginine 2 (R2me2a), a chromatin mark of silenced genes. Our structural model of the ternary complex reveals a distinct mode of dimethylarginine recognition, involving a polar interaction between R2me2a and its groove, the structural integrity of which is crucial for normal tissue patterning. Notably, humanized fly Pygo derepresses Notch targets, implying an inherent Notch-related function of classical Pygo orthologs, disabled in fly Pygo, which thus appears dedicated to Wnt signaling.


==About this Structure==
Evolutionary Adaptation of the Fly Pygo PHD Finger toward Recognizing Histone H3 Tail Methylated at Arginine 2.,Miller TC, Mieszczanek J, Sanchez-Barrena MJ, Rutherford TJ, Fiedler M, Bienz M Structure. 2013 Oct 30. pii: S0969-2126(13)00364-X. doi:, 10.1016/j.str.2013.09.013. PMID:24183574<ref>PMID:24183574</ref>
[[3zpv]] is a 36 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPV OCA].
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zpv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Bienz, M.]]
[[Category: Large Structures]]
[[Category: Fiedler, M.]]
[[Category: Bienz M]]
[[Category: Mieszczanek, J.]]
[[Category: Fiedler M]]
[[Category: Miller, T C.R.]]
[[Category: Mieszczanek J]]
[[Category: Rutherford, T J.]]
[[Category: Miller TCR]]
[[Category: Sanchez-Barrena, M J.]]
[[Category: Rutherford TJ]]
[[Category: Histone h3 tail binding]]
[[Category: Sanchez-Barrena MJ]]
[[Category: Transcription]]
[[Category: Wnt signaling pathway]]
[[Category: Zn finger]]
Retrieved from "https://proteopedia.org/3zpv"