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| [[Image:2pwt.gif|left|200px]]<br /><applet load="2pwt" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="2pwt, resolution 1.800Å" />
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| '''Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group'''<br />
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| ==Overview== | | ==Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group== |
| The crystal structure of the complex between oligonucleotide containing the bacterial ribosomal decoding site (A site) and the synthetic paromomycin analogue 1, which contains the gamma-amino-alpha-hydroxybutyryl (L-haba) group at position N1 of ring II (2-DOS ring), and an ether chain with an O-phenethylaminoethyl group at position C2'' of ring III, is reported. Interestingly, next to the paromomycin analogue 1 specifically bound to the A site, a second molecule of 1 with a different conformation is observed at the crystal packing interface which mimics the A-minor interaction between two bulged-out adenines from the A site and the codon-anticodon stem of the mRNA-tRNA complex. Improved antibacterial activity supports the conclusion that analogue 1 might affect protein synthesis on the ribosome in two different ways: 1) specific binding to the A site forces maintenance of the "on" state with two bulged out adenines, and 2) a new binding mode of 1 to an A-minor motif which stabilizes complex formation between the ribosome and the mRNA-tRNA complex regardless of whether the codon-anticodon stem is of the cognate or near-cognate type.
| | <StructureSection load='2pwt' size='340' side='right'caption='[[2pwt]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[2pwt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PWT FirstGlance]. <br> |
| 2PWT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=LHA:'>LHA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHA:DOUBLY+FUNCTIONALIZED+PAROMOMYCIN+PM-II-162'>LHA</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pwt OCA], [https://pdbe.org/2pwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pwt RCSB], [https://www.ebi.ac.uk/pdbsum/2pwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pwt ProSAT]</span></td></tr> |
| Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity., Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E, ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17722211 17722211]
| | </table> |
| [[Category: Protein complex]] | | __TOC__ |
| [[Category: Francois, B.]] | | </StructureSection> |
| [[Category: Hanessian, S.]] | | [[Category: Large Structures]] |
| [[Category: Kondo, J.]] | | [[Category: Francois B]] |
| [[Category: Pachamuthu, K.]] | | [[Category: Hanessian S]] |
| [[Category: Szychowski, J.]] | | [[Category: Kondo J]] |
| [[Category: Westhof, E.]] | | [[Category: Pachamuthu K]] |
| [[Category: LHA]]
| | [[Category: Szychowski J]] |
| [[Category: aminoglycoside; haba group; ribosomal decoding site; x-ray analysis; rna]]
| | [[Category: Westhof E]] |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:33:46 2008''
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