4i8b: Difference between revisions
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==Crystal Structure of Thioredoxin from Schistosoma Japonicum== | |||
<StructureSection load='4i8b' size='340' side='right'caption='[[4i8b]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4i8b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_japonicum Schistosoma japonicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4I8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4I8B FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4i8b OCA], [https://pdbe.org/4i8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4i8b RCSB], [https://www.ebi.ac.uk/pdbsum/4i8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4i8b ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9U8F3_SCHJA Q9U8F3_SCHJA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Schistosoma japonicum, a human blood fluke, causes a parasitic disease affecting millions of people in Asia. Thioredoxin-glutathione system of S. japonicum plays a critical role in maintaining the redox balance in parasite, which is a potential target for development of novel antischistosomal agents. Here we cloned the gene of S. japonicum thioredoxin (SjTrx), expressed and purified the recombinant SjTrx in Escherichia coli. Functional assay shows that SjTrx catalyses the dithiothreitol (DTT) reduction of insulin disulphide bonds. The coupling assay of SjTrx with its endogenous reductase, thioredoxin glutathione reductase from S. japonicum (SjTGR), supports its biological function to maintain the redox homeostasis in the cell. Furthermore, the crystal structure of SjTrx in the oxidized state was determined at 2.0 A resolution, revealing a typical architecture of thioredoxin fold. The structural information of SjTrx provides us important clues for understanding the maintenance function of redox homeostasis in S. japonicum and pathogenesis of this chronic disease. | |||
Expression, characterization and crystal structure of thioredoxin from Schistosoma japonicum.,Li Y, Li P, Peng Y, Wu Q, Huang F, Liu X, Li X, Zhou H, Guo D, Shi D, Zhou XN, Fan X Parasitology. 2015 Mar 26:1-9. PMID:25810021<ref>PMID:25810021</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4i8b" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Thioredoxin 3D structures|Thioredoxin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Schistosoma japonicum]] | |||
[[Category: Chen J]] | |||
[[Category: Fan X]] | |||
[[Category: Li X]] | |||
[[Category: Luo Z]] | |||
[[Category: Peng Y]] | |||
[[Category: Shi D]] | |||
[[Category: Wu Q]] | |||
[[Category: Zhao J]] | |||
[[Category: Zhou X]] | |||
Latest revision as of 03:01, 21 November 2024
Crystal Structure of Thioredoxin from Schistosoma Japonicum
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