2qlt: Difference between revisions

Latest revision as of 09:16, 21 February 2024

Crystal structure of an isoform of DL-glycerol-3-phosphatase, Rhr2p, from Saccharomyces cerevisiae

Structural highlights

2qlt is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Ligands:	CA, CL, EDO, HG, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

GPP1_YEAST Major isoform of glycerol-1-phosphate phosphohydrolase involved in glycerol biosynthesis. Plays a role in osmoadaptation and required for adaptation to anaerobic conditions.^[1] ^[2] ^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Norbeck J, Pahlman AK, Akhtar N, Blomberg A, Adler L. Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway. J Biol Chem. 1996 Jun 7;271(23):13875-81. PMID:8662716
↑ Pahlman AK, Granath K, Ansell R, Hohmann S, Adler L. The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stress. J Biol Chem. 2001 Feb 2;276(5):3555-63. Epub 2000 Oct 31. PMID:11058591 doi:https://dx.doi.org/10.1074/jbc.M007164200
↑ Nguyen HT, Dieterich A, Athenstaedt K, Truong NH, Stahl U, Nevoigt E. Engineering of Saccharomyces cerevisiae for the production of L-glycerol 3-phosphate. Metab Eng. 2004 Apr;6(2):155-63. PMID:15113568 doi:https://dx.doi.org/10.1016/j.ymben.2004.02.005
↑ Granath K, Modig T, Forsmark A, Adler L, Liden G. The YIG1 (YPL201c) encoded protein is involved in regulating anaerobic glycerol metabolism in Saccharomyces cerevisiae. Yeast. 2005 Dec;22(16):1257-68. PMID:16358322 doi:https://dx.doi.org/10.1002/yea.1307
↑ Popp A, Nguyen HT, Boulahya K, Bideaux C, Alfenore S, Guillouet SE, Nevoigt E. Fermentative production of L-glycerol 3-phosphate utilizing a Saccharomyces cerevisiae strain with an engineered glycerol biosynthetic pathway. Biotechnol Bioeng. 2008 Jun 15;100(3):497-505. doi: 10.1002/bit.21777. PMID:18438874 doi:https://dx.doi.org/10.1002/bit.21777

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OCA

[1] Norbeck J, Pahlman AK, Akhtar N, Blomberg A, Adler L. Purification and characterization of two isoenzymes of DL-glycerol-3-phosphatase from Saccharomyces cerevisiae. Identification of the corresponding GPP1 and GPP2 genes and evidence for osmotic regulation of Gpp2p expression by the osmosensing mitogen-activated protein kinase signal transduction pathway. J Biol Chem. 1996 Jun 7;271(23):13875-81. PMID:8662716

[2] Pahlman AK, Granath K, Ansell R, Hohmann S, Adler L. The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stress. J Biol Chem. 2001 Feb 2;276(5):3555-63. Epub 2000 Oct 31. PMID:11058591 doi:https://dx.doi.org/10.1074/jbc.M007164200

[3] Nguyen HT, Dieterich A, Athenstaedt K, Truong NH, Stahl U, Nevoigt E. Engineering of Saccharomyces cerevisiae for the production of L-glycerol 3-phosphate. Metab Eng. 2004 Apr;6(2):155-63. PMID:15113568 doi:https://dx.doi.org/10.1016/j.ymben.2004.02.005

[4] Granath K, Modig T, Forsmark A, Adler L, Liden G. The YIG1 (YPL201c) encoded protein is involved in regulating anaerobic glycerol metabolism in Saccharomyces cerevisiae. Yeast. 2005 Dec;22(16):1257-68. PMID:16358322 doi:https://dx.doi.org/10.1002/yea.1307

[5] Popp A, Nguyen HT, Boulahya K, Bideaux C, Alfenore S, Guillouet SE, Nevoigt E. Fermentative production of L-glycerol 3-phosphate utilizing a Saccharomyces cerevisiae strain with an engineered glycerol biosynthetic pathway. Biotechnol Bioeng. 2008 Jun 15;100(3):497-505. doi: 10.1002/bit.21777. PMID:18438874 doi:https://dx.doi.org/10.1002/bit.21777

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@@ Line 1: / Line 1: @@
-[[Image:2qlt.jpg|left|200px]]<br /><applet load="2qlt" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="2qlt, resolution 1.60&Aring;" />
-'''Crystal structure of an isoform of DL-glycerol-3-phosphatase, Rhr2p, from Saccharomyces cerevisiae'''<br />
-==About this Structure==
+==Crystal structure of an isoform of DL-glycerol-3-phosphatase, Rhr2p, from Saccharomyces cerevisiae==
-QLT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=HG:'>HG</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QLT OCA].
+<StructureSection load='2qlt' size='340' side='right'caption='[[2qlt]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2qlt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QLT FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qlt OCA], [https://pdbe.org/2qlt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qlt RCSB], [https://www.ebi.ac.uk/pdbsum/2qlt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qlt ProSAT], [https://www.topsan.org/Proteins/MCSG/2qlt TOPSAN]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GPP1_YEAST GPP1_YEAST] Major isoform of glycerol-1-phosphate phosphohydrolase involved in glycerol biosynthesis. Plays a role in osmoadaptation and required for adaptation to anaerobic conditions.<ref>PMID:8662716</ref> <ref>PMID:11058591</ref> <ref>PMID:15113568</ref> <ref>PMID:16358322</ref> <ref>PMID:18438874</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ql/2qlt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qlt ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Edwards A]]
-[[Category: Edwards, A.]]
+[[Category: Evdokimova E]]
-[[Category: Evdokimova, E.]]
+[[Category: Joachimiak A]]
-[[Category: Joachimiak, A.]]
+[[Category: Kudritska M]]
-[[Category: Kudritska, M.]]
+[[Category: Savchenko A]]
-[[Category: MCSG, Midwest Center for Structural Genomics.]]
+[[Category: Tan K]]
-[[Category: Savchenko, A.]]
-[[Category: Tan, K.]]
-[[Category: CA]]
-[[Category: CL]]
-[[Category: EDO]]
-[[Category: HG]]
-[[Category: SO4]]
-[[Category: apc7326]]
-[[Category: dl-glycerol-3-phosphatase isoform]]
-[[Category: hydrolase]]
-[[Category: mcsg]]
-[[Category: midwest center for structural genomics]]
-[[Category: protein structure initiative]]
-[[Category: psi-2]]
-[[Category: rhr2p]]
-[[Category: saccharomyces cerevisiae]]
-[[Category: structural genomics]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:40:19 2008''

2qlt: Difference between revisions

Latest revision as of 09:16, 21 February 2024

Crystal structure of an isoform of DL-glycerol-3-phosphatase, Rhr2p, from Saccharomyces cerevisiae

Structural highlights

Function

Evolutionary Conservation

References

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