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[[Image:2qp2.jpg|left|200px]]<br /><applet load="2qp2" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2qp2, resolution 2.000&Aring;" />
'''Structure of a MACPF/perforin-like protein'''<br />


==Overview==
==Structure of a MACPF/perforin-like protein==
Proteins containing membrane attack complex/perforin (MACPF) domains play important roles in vertebrate immunity, embryonic development, and neural-cell migration. In vertebrates, the ninth component of complement and perforin form oligomeric pores that lyse bacteria and kill virus-infected cells, respectively. However, the mechanism of MACPF function is unknown. We determined the crystal structure of a bacterial MACPF protein, Plu-MACPF from Photorhabdus luminescens, to 2.0 angstrom resolution. The MACPF domain reveals structural similarity with poreforming cholesterol-dependent cytolysins (CDCs) from Gram-positive bacteria. This suggests that lytic MACPF proteins may use a CDC-like mechanism to form pores and disrupt cell membranes. Sequence similarity between bacterial and vertebrate MACPF domains suggests that the fold of the CDCs, a family of proteins important for bacterial pathogenesis, is probably used by vertebrates for defense against infection.
<StructureSection load='2qp2' size='340' side='right'caption='[[2qp2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2qp2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_laumondii_subsp._laumondii Photorhabdus laumondii subsp. laumondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QP2 FirstGlance]. <br>
2QP2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Photorhabdus_luminescens_subsp._laumondii Photorhabdus luminescens subsp. laumondii] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QP2 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qp2 OCA], [https://pdbe.org/2qp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qp2 RCSB], [https://www.ebi.ac.uk/pdbsum/2qp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qp2 ProSAT]</span></td></tr>
A common fold mediates vertebrate defense and bacterial attack., Rosado CJ, Buckle AM, Law RH, Butcher RE, Kan WT, Bird CH, Ung K, Browne KA, Baran K, Bashtannyk-Puhalovich TA, Faux NG, Wong W, Porter CJ, Pike RN, Ellisdon AM, Pearce MC, Bottomley SP, Emsley J, Smith AI, Rossjohn J, Hartland EL, Voskoboinik I, Trapani JA, Bird PI, Dunstone MA, Whisstock JC, Science. 2007 Sep 14;317(5844):1548-51. Epub 2007 Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17717151 17717151]
</table>
[[Category: Photorhabdus luminescens subsp. laumondii]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q7N6X0_PHOLL Q7N6X0_PHOLL]
[[Category: Baran, K.]]
== Evolutionary Conservation ==
[[Category: Bashtannyk-Puhalovich, T A.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Bird, C H.]]
Check<jmol>
[[Category: Bird, P I.]]
  <jmolCheckbox>
[[Category: Bottomley, S P.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/2qp2_consurf.spt"</scriptWhenChecked>
[[Category: Browne, K A.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Buckle, A M.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Butcher, R E.]]
  </jmolCheckbox>
[[Category: Dunstone, M A.]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qp2 ConSurf].
[[Category: Ellisdon, A M.]]
<div style="clear:both"></div>
[[Category: Emsley, J.]]
__TOC__
[[Category: Faux, N G.]]
</StructureSection>
[[Category: Hartland, E L.]]
[[Category: Large Structures]]
[[Category: Kan, W T.]]
[[Category: Photorhabdus laumondii subsp. laumondii]]
[[Category: Law, R H.P.]]
[[Category: Baran K]]
[[Category: Pearce, M C.]]
[[Category: Bashtannyk-Puhalovich TA]]
[[Category: Pike, R N.]]
[[Category: Bird CH]]
[[Category: Porter, C J.]]
[[Category: Bird PI]]
[[Category: Rosado, C J.]]
[[Category: Bottomley SP]]
[[Category: Rossjohn, J.]]
[[Category: Browne KA]]
[[Category: Smith, A I.]]
[[Category: Buckle AM]]
[[Category: Trapani, J A.]]
[[Category: Butcher RE]]
[[Category: Ung, K.]]
[[Category: Dunstone MA]]
[[Category: Voskoboinik, I.]]
[[Category: Ellisdon AM]]
[[Category: Whisstock, J C.]]
[[Category: Emsley J]]
[[Category: Wong, W.]]
[[Category: Faux NG]]
[[Category: CA]]
[[Category: Hartland EL]]
[[Category: toxin]]
[[Category: Kan WT]]
[[Category: unknown function]]
[[Category: Law RHP]]
 
[[Category: Pearce MC]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:04 2008''
[[Category: Pike RN]]
[[Category: Porter CJ]]
[[Category: Rosado CJ]]
[[Category: Rossjohn J]]
[[Category: Smith AI]]
[[Category: Trapani JA]]
[[Category: Ung K]]
[[Category: Voskoboinik I]]
[[Category: Whisstock JC]]
[[Category: Wong W]]

Latest revision as of 13:54, 13 March 2024

Structure of a MACPF/perforin-like protein

2qp2, resolution 2.00Å

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