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[[Image:2qsf.jpg|left|200px]]<br /><applet load="2qsf" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2qsf, resolution 2.350&Aring;" />
'''Crystal structure of the Rad4-Rad23 complex'''<br />


==Overview==
==Crystal structure of the Rad4-Rad23 complex==
Mutations in the nucleotide excision repair (NER) pathway can cause the xeroderma pigmentosum skin cancer predisposition syndrome. NER lesions are limited to one DNA strand, but otherwise they are chemically and structurally diverse, being caused by a wide variety of genotoxic chemicals and ultraviolet radiation. The xeroderma pigmentosum C (XPC) protein has a central role in initiating global-genome NER by recognizing the lesion and recruiting downstream factors. Here we present the crystal structure of the yeast XPC orthologue Rad4 bound to DNA containing a cyclobutane pyrimidine dimer (CPD) lesion. The structure shows that Rad4 inserts a beta-hairpin through the DNA duplex, causing the two damaged base pairs to flip out of the double helix. The expelled nucleotides of the undamaged strand are recognized by Rad4, whereas the two CPD-linked nucleotides become disordered. These findings indicate that the lesions recognized by Rad4/XPC thermodynamically destabilize the Watson-Crick double helix in a manner that facilitates the flipping-out of two base pairs.
<StructureSection load='2qsf' size='340' side='right'caption='[[2qsf]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2qsf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QSF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qsf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qsf OCA], [https://pdbe.org/2qsf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qsf RCSB], [https://www.ebi.ac.uk/pdbsum/2qsf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qsf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RAD4_YEAST RAD4_YEAST] Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qs/2qsf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qsf ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2QSF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QSF OCA].
*[[DNA repair protein Rad4|DNA repair protein Rad4]]
 
*[[UV excision repair protein|UV excision repair protein]]
==Reference==
__TOC__
Recognition of DNA damage by the Rad4 nucleotide excision repair protein., Min JH, Pavletich NP, Nature. 2007 Oct 4;449(7162):570-5. Epub 2007 Sep 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17882165 17882165]
</StructureSection>
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Min, J H.]]
[[Category: Min J-H]]
[[Category: Pavletich, N P.]]
[[Category: Pavletich NP]]
[[Category: alpha-beta structure]]
[[Category: beta hairpin]]
[[Category: dna binding protein]]
[[Category: dna repair]]
[[Category: dna-damage recognition]]
[[Category: nucleotide excision repair]]
[[Category: transglutaminase fold]]
[[Category: xeroderma pigmentosum]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:41:55 2008''

Latest revision as of 09:17, 21 February 2024

Crystal structure of the Rad4-Rad23 complex

2qsf, resolution 2.35Å

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