4mz9: Difference between revisions

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{{STRUCTURE_4mz9|  PDB=4mz9  |  SCENE=  }}
===Revised structure of E. coli SSB===
{{ABSTRACT_PUBMED_10731701}}


==Function==
==Revised structure of E. coli SSB==
[[http://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI]] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.  
<StructureSection load='4mz9' size='340' side='right'caption='[[4mz9]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4mz9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MZ9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mz9 OCA], [https://pdbe.org/4mz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mz9 RCSB], [https://www.ebi.ac.uk/pdbsum/4mz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mz9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Single-stranded DNA (ssDNA) binding protein (SSB) is an essential protein to protect ssDNA and recruit specific ssDNA-processing proteins. Escherichia coli SSB forms a tetramer at neutral pH, comprising a structurally well-defined ssDNA binding domain (OB-domain) and a disordered C-terminal domain (C-domain) of approximately 64 amino acid residues. The C-terminal eight-residue segment of SSB (C-peptide) has been shown to interact with the OB-domain, but crystal structures failed to reveal any electron density of the C-peptide. Here we show that SSB forms a monomer at pH 3.4, which is suitable for studies by high-resolution nuclear magnetic resonance (NMR) spectroscopy. The OB-domain retains its 3D structure in the monomer, and the C-peptide is shown by nuclear Overhauser effects and lanthanide-induced pseudocontact shifts to bind to the OB-domain at a site that harbors ssDNA in the crystal structure of the SSB-ssDNA complex. 15N relaxation data demonstrate high flexibility of the polypeptide segment linking the C-peptide to the OB-domain and somewhat increased flexibility of the C-peptide compared with the OB-domain, suggesting that the C-peptide either retains high mobility in the bound state or is in a fast equilibrium with an unbound state.


==About this Structure==
Intramolecular binding mode of the C-terminus of Escherichia coli single-stranded DNA binding protein determined by nuclear magnetic resonance spectroscopy.,Shishmarev D, Wang Y, Mason CE, Su XC, Oakley AJ, Graham B, Huber T, Dixon NE, Otting G Nucleic Acids Res. 2013 Nov 27. PMID:24288378<ref>PMID:24288378</ref>
[[4mz9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MZ9 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:010731701</ref><references group="xtra"/><references/>
</div>
[[Category: Oakley, A J.]]
<div class="pdbe-citations 4mz9" style="background-color:#fffaf0;"></div>
[[Category: Dna binding protein]]
== References ==
[[Category: Dnag]]
<references/>
[[Category: Exoi]]
__TOC__
[[Category: Holc]]
</StructureSection>
[[Category: Reco]]
[[Category: Escherichia coli K-12]]
[[Category: Recq]]
[[Category: Large Structures]]
[[Category: Single strand dna-binding domain]]
[[Category: Oakley AJ]]
[[Category: Ssb]]
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