Sandbox Reserved 829: Difference between revisions
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[[Image:Schematic representation of functional domains and phosphorylation sites of PDX-1.jpg|right|580px|thumb|'''Schematic representation of functional domains and phosphorylation sites of PDX-1''']] | [[Image:Schematic representation of functional domains and phosphorylation sites of PDX-1.jpg|right|580px|thumb|'''Schematic representation of functional domains and phosphorylation sites of PDX-1''']] | ||
PDX-1 contains, at the N-terminus, a transactivation domain ( from 1 to 79 amino acids) and the middle region of the protein is composed of a homeodomain (from 146 to 206 amino acids) which is essential for DNA binding and protein-protein interactions. | PDX-1 contains, at the N-terminus, a transactivation domain (from 1 to 79 amino acids) and the middle region of the protein is composed of a homeodomain (from 146 to 206 amino acids) which is essential for DNA binding and protein-protein interactions. | ||
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===='''Motifs of the PDX-1 homeodomain''' | ===='''Motifs of the PDX-1 homeodomain''' [http://www.proteopedia.org/wiki/index.php/Image:Schematic_representation_of_functional_domains_and_phosphorylation_sites_of_PDX-1.jpg] ==== | ||
The homeodomain contains a Protein Transduction Domain (PTD : from 188 to 203 amino acids) and a Nuclear Localization Signal motif (NLS : from 197 to 203 amino acids), which allow PDX-1 to permeate into cells. | The homeodomain contains a Protein Transduction Domain (PTD : from 188 to 203 amino acids) and a Nuclear Localization Signal motif (NLS : from 197 to 203 amino acids), which allow PDX-1 to permeate into cells. | ||
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<ref name= "binding" />]] | <ref name= "binding" />]] | ||
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So, the most stable configuration for the N-terminal arm of Pdx1 consists of Lys 2 inserted in the minor groove and Arg 3 outside of the minor grove contacting the phosphate backbone and Arg 43. | So, the most stable configuration for the N-terminal arm of Pdx1 consists of Lys 2 inserted in the minor groove and Arg 3 outside of the minor grove contacting the phosphate backbone and Arg 43. | ||
