2ru4: Difference between revisions
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The | ==Designed Armadillo Repeat Protein Self-ASsembled Complex (YIIM2-MAII)== | ||
<StructureSection load='2ru4' size='340' side='right'caption='[[2ru4]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2ru4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RU4 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ru4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ru4 OCA], [https://pdbe.org/2ru4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ru4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ru4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ru4 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Repeat proteins are built of modules, each of which constitutes a structural motif. We have investigated whether fragments of a designed consensus armadillo repeat protein (ArmRP) recognize each other. We examined a split ArmRP consisting of an N-capping repeat (denoted Y), three internal repeats (M), and a C-capping repeat (A). We demonstrate that the C-terminal MA fragment adopts a fold similar to the corresponding part of the entire protein. In contrast, the N-terminal YM2 fragment constitutes a molten globule. The two fragments form a 1:1 YM2:MA complex with a nanomolar dissociation constant essentially identical to the crystal structure of the continuous YM3A protein. Molecular dynamics simulations show that the complex is structurally stable over a 1 mus timescale and reveal the importance of hydrophobic contacts across the interface. We propose that the existence of a stable complex recapitulates possible intermediates in the early evolution of these repeat proteins. | |||
Spontaneous self-assembly of engineered armadillo repeat protein fragments into a folded structure.,Watson RP, Christen MT, Ewald C, Bumbak F, Reichen C, Mihajlovic M, Schmidt E, Guntert P, Caflisch A, Pluckthun A, Zerbe O Structure. 2014 Jul 8;22(7):985-95. doi: 10.1016/j.str.2014.05.002. Epub 2014 Jun, 12. PMID:24931467<ref>PMID:24931467</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2ru4" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Christen MT]] | |||
[[Category: Plueckthun A]] | |||
[[Category: Watson RP]] | |||
[[Category: Zerbe O]] | |||
Latest revision as of 10:04, 14 June 2023
Designed Armadillo Repeat Protein Self-ASsembled Complex (YIIM2-MAII)
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