4o9p: Difference between revisions

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New page: '''Unreleased structure''' The entry 4o9p is ON HOLD Authors: Josephine H. Leung, Mutsuo Yamaguchi, Arne Moeller, Lici A. Schurig-Briccio, Robert B. Gennis, Clinton S. Potter, Bridget C...
 
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'''Unreleased structure'''


The entry 4o9p is ON HOLD
==Crystal structure of Thermus thermophilis transhydrogeanse domain II dimer SeMet derivative==
<StructureSection load='4o9p' size='340' side='right'caption='[[4o9p]], [[Resolution|resolution]] 2.89&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4o9p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O9P FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.89&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o9p OCA], [https://pdbe.org/4o9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o9p RCSB], [https://www.ebi.ac.uk/pdbsum/4o9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o9p ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q72GS0_THET2 Q72GS0_THET2] The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane (By similarity).[PIRNR:PIRNR000204]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
NADPH/NADP(+) (the reduced form of NADP(+)/nicotinamide adenine dinucleotide phosphate) homeostasis is critical for countering oxidative stress in cells. Nicotinamide nucleotide transhydrogenase (TH), a membrane enzyme present in both bacteria and mitochondria, couples the proton motive force to the generation of NADPH. We present the 2.8 A crystal structure of the transmembrane proton channel domain of TH from Thermus thermophilus and the 6.9 A crystal structure of the entire enzyme (holo-TH). The membrane domain crystallized as a symmetric dimer, with each protomer containing a putative proton channel. The holo-TH is a highly asymmetric dimer with the NADP(H)-binding domain (dIII) in two different orientations. This unusual arrangement suggests a catalytic mechanism in which the two copies of dIII alternatively function in proton translocation and hydride transfer.


Authors: Josephine H. Leung, Mutsuo Yamaguchi, Arne Moeller, Lici A. Schurig-Briccio, Robert B. Gennis, Clinton S. Potter, Bridget Carragher, Charles D. Stout
Structural biology. Division of labor in transhydrogenase by alternating proton translocation and hydride transfer.,Leung JH, Schurig-Briccio LA, Yamaguchi M, Moeller A, Speir JA, Gennis RB, Stout CD Science. 2015 Jan 9;347(6218):178-81. doi: 10.1126/science.1260451. PMID:25574024<ref>PMID:25574024</ref>


Description: Crystal structure of Thermus thermophilis transhydrogeanse domain II dimer SeMet derivative
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4o9p" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[NAD(P) transhydrogenase 3D structures|NAD(P) transhydrogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB27]]
[[Category: Carragher B]]
[[Category: Gennis RB]]
[[Category: Leung JH]]
[[Category: Moeller A]]
[[Category: Potter CS]]
[[Category: Schurig-Briccio LA]]
[[Category: Stout CD]]
[[Category: Yamaguchi M]]
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