Sandbox Reserved 824: Difference between revisions
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''Note : for a readability purpose the structures enlighten in the Jmol applet will focus only on one SRP54. The same structures are present in the second SRP54M of the dimer.'' | ''Note : for a readability purpose the structures enlighten in the Jmol applet will focus only on one SRP54. The same structures are present in the second SRP54M of the dimer.'' | ||
The secondary structure of | The secondary structure of human SRP54M is formed of <scene name='56/568022/Hsrp54m_h1_to_h7/1'>7 alpha helixes (H1 to H7)</scene>. The helices 2 to 7 form the <scene name='56/568022/Hsrp54m_core_and_h1v2/2'>Core structure</scene>, stabilized by hydrophobic, hydrogen and ionic interactions. | ||
Several residues important to maintain the Core structure were identified. Among them the <scene name='56/568022/Hsrp54m_core_and_h1/2'>Methionine 382,Glutamine 386, Arginine 402 and Arginine 405.</scene> | Several residues important to maintain the Core structure were identified. Among them the <scene name='56/568022/Hsrp54m_core_and_h1/2'>Methionine 382,Glutamine 386, Arginine 402 and Arginine 405.</scene> | ||
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SRP54M binds the SRP RNA 7S by electrostatic interactions. | SRP54M binds the SRP RNA 7S by electrostatic interactions. | ||
The residues responsible for this interaction are localized in <scene name='56/568022/Hsrp54m_rna_bindv3/1'>helices 4, 5, 6 and 7</scene>, but most particularly in helices 5 and 6. | The residues responsible for this interaction are localized in <scene name='56/568022/Hsrp54m_rna_bindv3/1'>helices 4, 5, 6 and 7</scene>, but most particularly in helices 5 and 6. | ||
A large part of the top is therefore positively charged to bind with the negatively charged 7S RNA. | A large part of the top of SRP54M is therefore positively charged to bind with the negatively charged 7S RNA. | ||
[[Image:Proteopedia charge.png|300px|center|thumb| The helices 4, 5, 6 and 7 surfaces are colored in blue on the spacefill model (left) of SRP54M in this picture. For more details see figure 7 of reference 1.]] | [[Image:Proteopedia charge.png|300px|center|thumb| The helices 4, 5, 6 and 7 surfaces are colored in blue on the spacefill model (left) of SRP54M in this picture. For more details see figure 7 of reference 1.]] | ||
